抗 谷胱甘肽-S-转移酶

该抗体对日本血吸虫 谷胱甘肽-S-转移酶的天然型和变性-还原型均具有特异性 。抗 GST 可用于各种免疫分析，以鉴定 GST 融合蛋白的表达。

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.

日本血吸虫 的重组 GST 在 大肠杆菌中表达 。

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

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