A8706
亲和素 来源于鸡蛋白
recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
别名:
AVID_CHICK
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关于此项目
Form:
powder
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in corn
Mol wt:
glycoprotein 66 kDa, subunit 16 kDa
recombinant
expressed in corn
assay
≥80% protein basis (biuret)
form
powder
specific activity
≥12 units/mg protein (E1%/280)
mol wt
glycoprotein 66 kDa, subunit 16 kDa
technique(s)
ELISA: suitable, immunoblotting: suitable, immunoelectrophoresis: suitable
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
chicken ... AVD(396260)
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General description
亲和素是一种同源四聚体糖蛋白，存在于鸟类、爬行动物和两栖动物的蛋清中。每个亚基为16 kDa，单糖基化并以更大的亲和力和特异性与生物素分子结合。在等电点（pI）和抗原特性（除了分子量）方面，玉米来源的重组抗生物素蛋白与鸡蛋来源的抗生物素蛋白相似。玉米来源的抗生物素蛋白比鸡蛋来源的抗生物素蛋白的分子量低。这可能是由于植物中糖基化组成较不复杂。从玉米中商业化生产抗生物素蛋白在可获得更大的生物量和避免动物病毒的共纯化方面具有一定的优势。
Application
亲和素与d-生物素（Kd ~ 10-15）形成极强的复合物。这种活性使抗生物素蛋白和生物素在检测和定量的免疫化学方法中都非常有用。
亲和素蛋白用于一项分析中，该分析使用官能化氙作用检测生物素-亲和素结合的生物传感器。使用80nmol的鸡蛋白。
Preparation Note
亲和纯化
Other Notes
1 个单位能够结合1.0 μg d-生物素。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物来源生物产品
此项目有
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The avidin-biotin complex (ABC) method and other avidin-biotin binding methods.
G L Bratthauer
Methods in molecular biology (Clifton, N.J.), 115, 203-214 (1999-03-31)
Ray C Schmidt et al.
Biomaterials, 34(15), 3758-3762 (2013-03-05)
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