A8811
醛缩酶 来源于兔肌肉
ammonium sulfate suspension, 10-20 units/mg protein
别名:
D-果糖-1,6-二磷酸-D-甘油醛-3-磷酸裂解酶, 二磷酸果糖酶
产品名称
醛缩酶 来源于兔肌肉, ammonium sulfate suspension, 10-20 units/mg protein
biological source
rabbit muscle
form
ammonium sulfate suspension
specific activity
10-20 units/mg protein
foreign activity
glyceraldehyde-3-phosphate dehydrogenase ≤0.03%
lactic dehydrogenase ≤0.03%
phosphoglucose isomerase ≤0.6%
pyruvate kinase ≤0.1%
triosephosphate isomerase ≤0.05%
storage temp.
2-8°C
Quality Level
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Analysis Note
由缩二脲测定的蛋白质。
Application
兔肌肉醛缩酶已用于：
- 在标准兔肌肉来源1-磷酸果糖激酶 (RPFK-1)实验中使用
- 作为青光眼组织来源代谢酶测定的标准品
- 用于人细胞系的果糖2,6-二磷酸分析
醛缩酶可用于将果糖1,6-二磷酸转化为磷酸二羟丙酮和3-磷酸甘油醛。来自兔肌肉中的醛缩酶已被用于DHAP C3的立体特异性去质子化。
Biochem/physiol Actions
醛缩酶与Wiskott-Aldrich综合征蛋白(WASP)相互作用，可调节肌动蛋白动态。它可逆转抗坏血酸对肌肉型乳酸脱氢酶(LDH-m4)的抑制作用。
醛缩酶可参与到糖原异生、卡尔文循环和糖酵解。来自兔肌肉中的醛缩酶属于I类醛缩酶，可形成共价席夫碱中间体。醛缩酶的活性位点位于α/β 8 筒形褶皱 的中央。
General description
兔醛缩酶存在三种亚型，包括来自肌肉的A型、肝脏B型和大脑相关C型。醛缩酶分子量为158 kDa，以四聚体形成存在。
Other Notes
在pH 7.4、25 °C条件下，一个单位可在每分钟内将1.0 μ摩尔的的果糖1,6-二磷酸转化为磷酸二羟丙酮和3-磷酸甘油醛。
Physical form
2.5M (NH4)2SO4、0.01M Tris 溶液中的结晶悬浮液，pH 7.5，含 0.001M EDTA
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein
St-Jean M, et al.
The Journal of biological chemistry, 282(19), 14309-14315 (2007)
Structure of rabbit muscle aldolase at low resolution.
Sygusch J, et al.
The Journal of Biological Chemistry, 260(28), 15286-15290 (1985)
Effect of lithium salts on lactate dehydrogenase, adenylate kinase, and 1-phosphofructokinase activities
Russell P, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 25(4), 551-556 (2010)
Clotilde LowKam et al.
The Journal of biological chemistry, 285(27), 21143-21152 (2010-04-30)
Tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes is a class I aldolase that exhibits a remarkable lack of chiral discrimination with respect to the configuration of hydroxyl groups at both C3 and C4 positions. The enzyme catalyzes the reversible cleavage of four
Structure- and ligand-based structure-activity relationships for a series of inhibitors of aldolase.
Leonardo G Ferreira et al.
Current computer-aided drug design, 8(4), 309-316 (2012-06-28)
Aldolase has emerged as a promising molecular target for the treatment of human African trypanosomiasis. Over the last years, due to the increasing number of patients infected with Trypanosoma brucei, there is an urgent need for new drugs to treat
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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