产品名称
抗生物素蛋白 −琼脂糖类 来源于鸡蛋白, aqueous glycerol suspension
biological source
egg white
conjugate
agarose conjugate
form
aqueous glycerol suspension
matrix
cross-linked 6% beaded agarose
matrix activation
epichlorohydrin
matrix attachment
amino
matrix spacer
15 atoms
storage temp.
−20°C
Quality Level
Application
亲和素用于含生物素蛋白的纯化，与对照人免疫球蛋白 (IgG) 功能化胶囊相比，亲和素可增强抗体 (Ab) 功能化胶囊的细胞结合和内化，表明这些胶囊可通过抗体/抗原识别与癌细胞发生特异性相互作用。
来自蛋清的抗生物素蛋白-琼脂糖已用于：
- 在成神经细胞瘤细胞系 (SH-SY5Y) 中通过下拉试验沉淀生物素标记蛋白
- 在纯化 曼氏血吸虫的 Lewis (x) 糖结合蛋白中
- 在抗生物素蛋白微珠试验中纯化标记生物素
- 从鼠角膜样品中捕获糖蛋白
Biochem/physiol Actions
亲和素由于其高亲和力的配体结合特性而得到生物技术的应用。亲和素通过其碳水化合物链与生物素相互作用在生物技术应用中被广泛利用。在癌细胞中，抗生物素蛋白-生物素系统有助于在肿瘤细胞中的体内定位和药物靶向。
General description
抗生物素蛋白占总蛋清蛋白的 0.05%。是一种糖基化蛋白。相当于分子量为 16.5 kDa，以四聚体形式存在，可结合至少 3 个生物素分子。
Other Notes
1 个单位能够结合 1.0 μg d-生物素。
Physical form
50% 甘油、0.01 M 磷酸钠 (pH 7.0) 混悬液，含 0.02% 叠氮化钠。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
Hytonen VP, et al.
The Biochemical Journal, 384(2), 385-390 (2004)
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The current consensus is that environmental electrophiles activate redox signal transduction pathways through covalent modification of sensor proteins with reactive thiol groups at low concentrations, while they cause cell damage at higher concentrations. We previously exposed human carcinoma A431 cells
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Molecular immunology, 38(12-13), 911-919 (2002-05-16)
Fucosylated sugars in Schistosoma mansoni possess immunomodulatory properties. In order to gain insights to the mechanisms involved, attempts were made to identify host immune cell molecules that specifically recognize these sugars. On Western blots, specific binding of synthetic biotinylated fucose
Corneal surface glycosylation is modulated by IL-1R and Pseudomonas aeruginosa challenge but is insufficient for inhibiting bacterial binding
Jolly AL, et al.
Faseb Journal, 31(6), 2393-2404 (2017)
Amber L Jolly et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(6), 2393-2404 (2017-02-23)
Cell surface glycosylation is thought to be involved in barrier function against microbes at mucosal surfaces. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to
相关内容
利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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