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Merck
CN

A9469

单克隆抗-FLAG®碱性磷酸酶 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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关于此项目

NACRES:
NA.32
UNSPSC Code:
41106514
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产品名称

单克隆抗-FLAG®碱性磷酸酶 小鼠抗, clone M2, purified immunoglobulin, buffered aqueous glycerol solution

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Quality Level

Gene Information

human ... ALPL(249)

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Application

小鼠中产生的单克隆 ANTI-FLAG® M2-碱性磷酸酶抗体已用于:
  • 甜橙叶柄样品的直接组织印迹免疫测定
  • δ阿片受体内化的筛选
  • 采用 ELISA 筛查无细胞的蛋白表达

成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质印迹(1篇论文)

General description

单克隆ANTI-FLAG® M2碱性磷酸酶是一种纯化的 IgG 1 小鼠抗体,与小牛肠碱性磷酸酶(AP)共价结合。该抗体偶联物可与FLAG®融合蛋白结合,并在融合蛋白的任何位置(N末端、Met-N末端、C末端或内部FLAG®肽)识别FLAG®表位。

Physical form

在含有50%甘油以及稳定剂和防腐剂的磷酸盐缓冲盐溶液中

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number

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Beth A Rasala et al.
Plant biotechnology journal, 9(6), 674-683 (2011-05-04)
Microalgae have the potential to be a valuable biotechnological platform for the production of recombinant proteins. However, because of the complex regulatory network that tightly controls chloroplast gene expression, heterologous protein accumulation in a wild-type, photosynthetic-competent algal chloroplast remains low.
Seongjoon Kang et al.
Biology, 7(2) (2018-05-09)
Chlamydomonas reinhardtii (Chlamydomonas) strains that are toxic to mosquito larvae because they express chloroplast transgenes that are based on the mosquitocidal proteins of Bacillus thuringiensis subsp. israelensis (Bti) could be very useful in mosquito control. Chlamydomonas has several advantages for
Jiandong Chen et al.
Genes & development, 31(13), 1382-1395 (2017-08-11)
Mismatch repair (MMR) is a conserved mechanism exploited by cells to correct DNA replication errors both in growing cells and under nongrowing conditions. Hfq (host factor for RNA bacteriophage Qβ replication), a bacterial Lsm family RNA-binding protein, chaperones RNA-RNA interactions
Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA
Layton CJ and Helling HW
Protein Science, 20(8), 1432-1438 (2011)
Waithaka Mwangi et al.
Journal of leukocyte biology, 78(2), 401-411 (2005-04-29)
Induction of immune responses against microbial antigens using DNA is an attractive strategy to mimic the immunity induced by live vaccines. Although DNA vaccines are efficacious in murine models, the requirement for multiple immunizations using high doses in outbred animals

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