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Merck
CN

A9570

MilliShot® Amphotericin B Ready Made Solution

250 μg/mL in deionized water, 0.2 μm filtered, BioReagent, suitable for cell culture

别名:

Amphotericin, Fungizone

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NACRES:
NA.76
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biological source

yeast

Quality Segment

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

shelf life

2 yr

storage condition

protect from light

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

-10 to -25°C

General description

Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain

Application

Amphotericin B solution has been used:
  • as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice
  • as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries
  • as a constituent of transport medium during the collection of equine corpora lutea
  • as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line

Biochem/physiol Actions

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium. Amphotericin B is most effective against fungi and yeast. It forms transmembrane channels by binding to sterols associated with membranes, thereby leading to the leakage of small molecules. Amphotericin B elicits toxicity despite being an effective antibiotic for treating mycoses.

Legal Information

MILLISHOT is a registered trademark of Merck KGaA, Darmstadt, Germany


存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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