Application
在一项评估铬代谢性酸中毒的响应过程中肾氨转运体表达情况的研究中，用户使用了Sigma氨检测试剂盒来测定血清和尿液中的氨浓度。
本试剂盒可用于酶促定量分析、测定食品及其他生物材料中的氨水平。 L-谷氨酸脱氢酶(GIDH)存在的情况下，氨能够与α-酮戊二酸(KGA)和还原型烟酰胺腺嘌呤二核苷酸磷酸盐(NADPH)反应，生成L-谷氨酸和氧化型烟酰胺腺嘌呤二核苷酸磷酸盐(NADP+)。340 nm波长处吸光值下降是NADPH的氧化所致，具体的吸光度与样品中的氨浓度成正比。 L-谷氨酸脱氢酶能够与氨特异性地反应。氨检测试剂盒可用于测定0.2-15 μg/ml的氨浓度。
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仅试剂盒组分
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说明
- Ammonia Assay Reagent 10 vial(s)
- L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
- Ammonia Standard Solution 1 mL/vial
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.
本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物，但许多肿瘤细胞表现出线粒体活性增加。
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Instructions
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Mammalian-inducible expression systems are increasingly available and offer an attractive platform for the production of recombinant proteins. In this work, we have conducted process development for a cumate-inducible GS-CHO cell-line-expressing rituximab. To cope with the limitations encountered in batch when
Na Gao et al.
Fish & shellfish immunology, 65, 226-234 (2017-04-22)
Ammonia is highly toxic to aquatic organisms, but whether ammonia excretion or ammonia metabolism to less toxic compounds is the major strategy for detoxification in marine fish against chronic ammonia exposure is unclear to date. In this study, we investigated
G C Santos et al.
Scientific reports, 7(1), 9510-9510 (2017-08-27)
We combined
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| AA0100-1KT | 04061833418369 |