Alkaline Phosphatase Blue Microwell Substrate

Name: Alkaline Phosphatase Blue Microwell Substrate
Brand: SIGMA

alkaline phosphatase substrate, solution

别名:

5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays

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About This Item

UNSPSC代码:

12352204

NACRES:

NA.83

Product Name

Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate

表单

solution

质量水平

200

用途

sufficient for 400 mL working substrate

储存温度

2-8°C

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一般描述

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

应用

Prior to reaction with alkaline phosphatase, the BCIP^® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

外形

A 2 component kit sufficient for preparing 400 mL of working substrate.

Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)