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Merck
CN

AB0100

Alkaline Phosphatase Blue Microwell Substrate

alkaline phosphatase substrate, solution

别名:

5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays

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关于此项目

UNSPSC Code:
12352204
NACRES:
NA.83
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产品名称

Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate

form

solution

Quality Segment

usage

sufficient for 400 mL working substrate

storage temp.

2-8°C

General description

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

Application

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany


存储类别

10 - Combustible liquids



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