表单
liquid
包装
pkg of 250 mL (AC-60250 (HighPrep™ PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep™ PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep™ PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep™ PCR (500 mL)))
储存条件
do not freeze
储存温度
2-8°C
一般描述
Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
应用
HighPrep™ PCR Clean-up System may be used in:
- Post-PCR and post-enzymatic reaction clean-up
- NGS library preparation
- Amplicon size selection for NGS
- Microarrays
- PCR
- Sanger sequencing
- Restriction enzyme digestions, adapter ligations
- Cloning
特点和优势
- High recovery of >100 bp amplicons
- Adaptable to high throughput liquid handling systems
- Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
- No centrifugation or filtration leads to higher and purer yields
制备说明
14 months when stored as directed
法律信息
HighPrep is a trademark of MagBio Genomics, Inc.
免责声明
For Research Use Only. Not for Use in Diagnostic Procedures.
警示用语：
Warning
危险声明
危险分类
Repr. 2
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Soil bacterial diversity under conservation agriculture-based cereal systems in Indo-Gangetic Plains
Choudhary M, et al.
3 Biotech, 8, 304-304 (2018)
Madhu Choudhary et al.
3 Biotech, 8(7), 304-304 (2018-07-14)
In Indo-Gangetic plains (IGP) of India, natural resources (soil, water, and environment) are degrading under the conventional-till (CT)-based management practices in rice-wheat cropping system. A long-term field experiment was conducted to understand the soil bacterial diversity and abundance under different
Deep sequencing of near full-length HIV-1 genomes from plasma identifies circulating subtype C and infrequent occurrence of AC recombinant form in Southern India}
Alampalli SV, et al.
PLoS ONE, 17 (2017)
Signature of genome wide gene expression in classical swine fever virus infected macrophages and PBMCs of indigenous vis-a-vis crossbred pigs
Sah V, et al
Gene, 144356-144356 (2020)
N Salvetat et al.
Journal of neurovirology, 25(6), 825-836 (2019-07-25)
Treatment-emergent depression is a common complication in patients with chronic hepatitis C virus (HCV) infection undergoing antiviral combination therapy with IFN-α and ribavirin. It has recently been shown that changes in A-to-I RNA editing rates are associated with various pathologies
实验方案
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持