一般描述
碱性磷酸酶二乙醇胺检测试剂盒提供了一种检测碱性磷酸酶活性的即用型试剂。这种简单的检测分析碱性磷酸酶活性的方法使用了p-磷酸对硝基苯酯（pNPP）作为底物。
应用
碱性磷酸酶活性二乙醇胺法检测试剂盒已被用于：
- 检测血清和成骨细胞中碱性磷酸酶（ALP）的水平
- 监测小鼠成肌细胞C2C12细胞在成骨细胞分化过程中的ALP水平
- 测定肺癌骨转移模型中的ALP活性
- 测定结肠组织匀浆中的ALP水平
适用于多种样品中碱性磷酸酶活性的测定。
生化/生理作用
碱性磷酸酶将pNPP水解为对硝基苯酚和无机磷酸盐。在37°C下孵育碱性磷酸酶样品和底物期间，通过监测405 nm处吸光度的增加来追踪反应。
警示用语：
Danger
危险分类
Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
靶器官
Kidney,Liver,Blood
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
XiaoQing Lu et al.
Medical science monitor : international medical journal of experimental and clinical research, 25, 9524-9530 (2019-12-14)
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