usage
(20 tests)
Quality Level
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
General description
Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞，并使用流式细胞仪进行定量。AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯（FITC）偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。该试剂盒包含碘化丙啶(PI)，用于标记细胞膜完全破坏的坏死细胞内的DNA。这种组合可以区分早期凋亡细胞（annexin V阳性, PI阴性），坏死细胞（annexin V阳性, PI阳性），以及活细胞（annexin V阴性, PI阴性）。
Application
Annexin V-FITC 凋亡检测试剂盒适于以下应用：
- 在治疗前列腺癌的过程中对LNCaP前列腺癌细胞染色以测定 G. lucidum提取物活性。
- 在研究DBP-maf（维生素D结合蛋白-巨噬细胞活化因子）基因对前列腺癌细胞活性的抑制中，用于对肿瘤细胞的标记。
- 用于对flippase酶活性的间接测定。
Features and Benefits
相比基于DNA的检测法（比如TUNEL法），可检测到细胞凋亡的更早期进程。
- 快速标记细胞。细胞染色仅需10分钟。
- 细胞无需固定或预处理，节省检测时间并满足细胞用于进一步研究。
- 试剂盒内含碘化丙啶二级染料，用于从活细胞和坏死细胞中区分出凋亡细胞。
Other Notes
使用前使所有组分达到室温。
仅试剂盒组分
产品编号
说明
- APOAFA
存储类别
10 - Combustible liquids
法规信息
常规特殊物品
此项目有
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细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| APOAF-20TST | 04061835568147 |
| APOAF-50TST | 04061835509607 |
| APOAF-60TST | 04061841682462 |
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