B0939
活性BAFF 人
Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)
别名:
B lymphocyte stimulator, B-cell-activating factor BAFF, BLyS, Dendritic cell-derived TNF-like molecule, TALL-1, TNF- and APOL-related leukocyte expressed ligand 1
并加购，请在购物车里查看协议价、货期和发货地。
选择规格
变更视图
关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in Nicotiana
Assay:
>97% (SDS-PAGE)
Form:
lyophilized
Mol wt:
18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids)
Impurities:
Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
biological source
human
Quality Segment
recombinant
expressed in Nicotiana
assay
>97% (SDS-PAGE)
form
lyophilized
potency
≤50 ng/mL ED50
mol wt
18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids)
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TNFSF13B(10673)
General description
BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.
Physical form
Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.
Preparation Note
Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.
Analysis Note
The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED50 ≤ 50 ng/mL
*activity results may vary with PBMC donors.
ED50 ≤ 50 ng/mL
Other Notes
Extinction Coefficient: E0.1% = 0.791 (A280 nm)
p.I: 6.03
p.I: 6.03
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable