描述
Sulfhydryl specific, water soluble biotinylation reagent
质量水平
表单
powder
溶解性
H2O: 10 mg/mL
储存温度
2-8°C
SMILES字符串
ICC(=O)NCCOCCOCCNC(=O)CCCCC1SCC2NC(=O)NC12
InChI
1S/C18H31IN4O5S/c19-11-16(25)21-6-8-28-10-9-27-7-5-20-15(24)4-2-1-3-14-17-13(12-29-14)22-18(26)23-17/h13-14,17H,1-12H2,(H,20,24)(H,21,25)(H2,22,23,26)
InChI key
LWGLKVINXNMNJX-UHFFFAOYSA-N
相关类别
一般描述
生物素聚环氧乙烷（PEO）碘乙酰胺是巯基（硫醇）特异性生物素化试剂。
应用
- 包含一个 12 原子亲水间隔区。
- pH 7.5-8.5 通常与巯基偶联
- 在许多蛋白质组学类型应用（如肽图和质谱）中，半胱氨酸标记尤其有用。
生物素聚氧化乙烯碘乙酰胺已被用作巯基反应试剂，以证明人乳头瘤病毒 16 的衣壳蛋白 L1 中存在游离巯基。已用于 ELISA（酶联免疫吸附试验）竞争和牵出试验的蛋白生物素化。
生化/生理作用
生物素聚环氧乙烷（PEO）碘乙酰胺基团在pH 7.5-8.5下与还原的硫醇发生特异性反应。含有巯基的肽和小分子也可用该试剂标记。生物素PEO碘乙酰胺能够标记半胱氨酸残基并与蛋白质中的巯基偶联，从而使其可用于蛋白质组学、肽图分析、磷酸肽分析和质谱分析。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
Beta-peptides with improved affinity for hDM2 and hDMX.
Harker EA, et al.
Bioorganic & Medicinal Chemistry, 17, 2038-2046 (2009)
Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1.
Ishii Y, et al.
Virology Journal, 4, 110-110 (2007)
A thermodynamic model for Nap1-histone interactions.
Andrews AJ, et al.
The Journal of Biological Chemistry, 283, 32412-32418 (2008)
Eric A Berg et al.
Cold Spring Harbor protocols, 2020(1), pdb-pdb (2020-01-04)
There are several techniques for biotinylating antibodies, from the most basic (using NHS-ester biotin to label primary amines) to more complex experiments (modifying sulfhydryls and carbohydrates). Biotinylation of free sulfhydryls, described here, can be effectively mediated using haloacetyl biotin derivatives.
Barbara Roman et al.
Journal of molecular and cellular cardiology, 144, 87-96 (2020-05-23)
In cardiomyocytes, there is microRNA (miR) in the mitochondria that originates from the nuclear genome and matures in the cytoplasm before translocating into the mitochondria. Overexpression of one such miR, miR-181c, can lead to heart failure by stimulating reactive oxygen
商品
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