B3679
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
别名:
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system
应用
BCIP®/NBT-紫色液体膜底物系统已被用作碱性磷酸酶的底物
- 用于通过免疫印迹法检测二抗
- 用于ELISPOT测定分析
- 用于斑点印迹分析
- 用于免疫印迹分析，以可视化硝酸纤维素膜上的蛋白条带
生化/生理作用
5-溴-4-氯-3-吲哚基磷酸酯 (BCIP)/硝基蓝四唑（NBT）/硝基蓝四唑鎓(NBT)是用于检测碱性磷酸酶(AP)的显色底物系统。AP水解BCIP形成中间体无色靛蓝化合物，并进一步被NBT氧化形成5,5′-二溴-4,4′-二氯靛蓝(BCI)。其中，NBT被还原为不溶的紫色双甲双胍(DF)，形成NBT-DF。NBT-DF和BCI的混合物形成深紫色沉淀。AP可水解多种BCIP分子，因此成为了一种高灵敏度方法。
外形
即用型。
法律信息
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
在2-8 °C稳定至少一年。
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Extracellular vesicles (EV) can transfer cellular molecules for specific intercellular communication with potential relevance in pathological conditions. We searched for the presence in plasma from coronary artery disease (CAD) patients of EV containing the adenosine A2A receptor (A2A R), a
T W J M Van Herpen et al.
Annals of botany, 102(3), 331-342 (2008-07-16)
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Characterization of the 41 kDa allergen Asp v 13, a subtilisin-like serine protease from Aspergillus versicolor
Shi C and Miller JD
Molecular Immunology, 48(15-16), 1827-1834 (2011)
Hailong Qi et al.
Vaccines, 10(3) (2022-03-27)
SARS-CoV-2 spike (S) variants that may evade antibody-mediated immunity are emerging. Evidence shows that vaccines with a stronger immune response are still effective against mutant strains. Here, we report a targeted type 1 conventional dendritic (cDC1) cell strategy for improved
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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