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Merck
CN

B4186

Sigma-Aldrich

丁酰胆碱酯酶 来源于人类血清

vial of ≥4 units

别名:

假性胆碱酯酶, 胆碱酯酶,丁酰基, 酰基胆碱酰基水解酶

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

形式

powder

质量水平

比活

≥50 units/mg protein

包装

vial of ≥4 units

UniProt登记号

储存温度

2-8°C

基因信息

human ... BCHE(590)

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应用

人丁酰胆碱酯酶已被用于研究其作为阿尔茨海默′病 (AD) 体内病理特征调节剂的作用。
来自西格玛的BchE已被用于通过各种测试化合物测定丁酰胆碱酯酶抑制活性

生化/生理作用

丁酰胆碱酯酶(BChE)是一种丝氨酸水解酶,其与乙酰胆碱酯酶(AchE)具有显著的结构相似性,但具有不同的底物和抑制剂特异性。 它存在于脊椎动物的血清、造血细胞、肝脏、肺脏、心脏和中枢神经系统中。BChE可催化胆碱的大型酯,如丁酰胆碱和苯甲酰胆碱。BChE活性的选择性抑制可用于有机磷酸酯的检测。BchE可用于治疗有机磷酸酯毒性。它是阿尔茨海默′病 (AD) 治疗中的关键靶点。
丁酰胆碱酯酶(BChE)是一种丝氨酸水解酶,其与乙酰胆碱酯酶(AchE)具有显著的结构相似性,但具有不同的底物和抑制剂特异性。BChE存在于脊椎动物的血清、造血细胞、肝脏、肺脏、心脏和中枢神经系统中。

单位定义

在pH8.0,37℃条件下,一个单位每分钟可将1.0μmole丁酰胆碱水解为胆碱和丁酸盐。使用丁酰胆碱作为底物获得的活性是使用乙酰胆碱作为底物所获得的活性的约2.5倍。

分析说明

经单独供体测试的起始材料,并经发现对HIV I和II抗体、乙型肝炎表面抗原和丙型肝炎抗体呈阴性。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What amount of enzyme is present in 1 vial of Product B4186, Butyrylcholinesterase from human serum?

    Each vial will contain at least 5 units. The vial content is given on the certificate of analysis of each lot. The vial may be substantially overpacked.

  6. How should Product B4186, Butyrylcholinesterase from human serum, be reconstituted?

    The enzyme can be reconstituted in 20 mM sodium phosphate buffer, pH 7.0, with 0.15 M sodium chloride.

  7. Are any buffer salts present in Product B4186, Butyrylcholinesterase from human serum?

    This product was lyophilized from 20 mM ammonium bicarbonate, which is a volatile buffer. So it would be expected to contain only traces of buffer salts.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Cholinesterases and engineered mutants for the detection of organophosphorus pesticide residues
Xu YL, et al.
Sensors, 18(12), 4281-4281 (2018)
The apolipoprotein E varepsilon4 allele plays pathological roles in AD through high protein expression and interaction with butyrylcholinesterase
Darreh-Shori T, et al.
Neurobiology of Aging, 32(7), 1236-1248 (2011)
Butyrylcholinesterase: an important new target in Alzheimer's disease therapy
Greig NH, et al.
International Psychogeriatrics / IPA, 14(S1), 77-91 (2002)
Huan-Huan Li et al.
Journal of enzyme inhibition and medicinal chemistry, 37(1), 2099-2111 (2022-07-29)
Novel scaffolds are expected to treat Alzheimer's disease, pyrazole-5-fluorosulfates were found as selective BuChE inhibitors. Compounds K1-K26 were assayed for ChE inhibitory activity, amongst them, compound K3 showed potent BuChE and hBuChE inhibition (IC50 = 0.79 μM and 6.59 μM). SAR analysis
Chengyao Wu et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 1860-1873 (2021-08-25)
To discover novel scaffolds as leads against dementia, a series of δ-aryl-1,3-dienesulfonyl fluorides with α-halo, α-aryl and α-alkynyl were assayed for ChE inhibitory activity, in which compound A10 was identified as a selective BuChE inhibitor (IC50 = 0.021 μM for eqBChE

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