B4679
BIS-TRIS丙烷
BioPerformance Certified, suitable for cell culture, ≥99.0%
别名:
1,3-双[三(羟甲基)甲氨基]丙烷
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线性分子式:
CH2[CH2NHC(CH2OH)3]2
化学文摘社编号:
分子量:
282.33
Beilstein:
1786109
EC 号:
MDL编号:
UNSPSC代码:
12161700
PubChem化学物质编号:
NACRES:
NA.25
等级
BioPerformance Certified
质量水平
方案
≥99.0%
表单
crystals
技术
cell culture | mammalian: suitable
杂质
endotoxin and bioburden, tested
有效pH范围
6.3-9.5
pKa (25 °C)
(1) 6.8, (2) 9.0
mp
164-165 °C (lit.)
应用
diagnostic assay manufacturing
异质活性
DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
SMILES字符串
OCC(CO)(CO)NCCCNC(CO)(CO)CO
InChI
1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
InChI key
HHKZCCWKTZRCCL-UHFFFAOYSA-N
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应用
- 中和的嵌合DNA探针改善纳米线场效应晶体管中富含GC RNA的检测。: 在纳米线场效应晶体管的开发过程中，研究人员使用BIS-TRIS丙烷来检测富含GC RNA。该研究证实了缓冲液′在增进这些生物传感器特异性和灵敏度方面的作用 （Chou et al., 2019）。
- 通过优化用于测定重组活化凝血因子VII药物产品中无γ-羧基谷氨酸（Gla）结构域杂质的阴离子交换色谱法来消除鬼峰（ghost peak）: 在优化色谱法的过程中研究人员使用BIS-TRIS丙烷来消除药物杂质分析中的鬼峰。该缓冲液的′有效性确保了药物制剂中杂质准确可靠的检测（Tahmasbi et al., 2019）。
其他说明
很容易将 BIS-TRIS 丙烷产品规格与 BIS-TRIS 丙烷规格表进行比较。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
Michael C Breadmore
Journal of chromatography. A, 1217(24), 3900-3906 (2010-05-11)
An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could
S J Smith et al.
The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)
The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)
R Coronado et al.
The Journal of general physiology, 79(4), 529-547 (1982-04-01)
A collection of organic cations has been used to probe the gross structural features of the ionic diffusion pathway in a K+-selective channel from sarcoplasmic reticulum (SR). Channels were incorporated into planar phospholipid bilayer membranes, and single-channel currents were measured
Sarita V Mello et al.
Biomacromolecules, 4(4), 968-973 (2003-07-15)
In this paper, we describe the preparation and characterization of Langmuir and Langmuir-Blodgett (LB) monolayers of the enzyme organophosphorus acid anhydrolase (OPAA). Langmuir films of OPAA were characterized on different subphases, such as phosphate, ammonium carbonate, and bis-tris-propane buffers. Monolayers
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