B4679
BIS-TRIS丙烷
BioPerformance Certified, suitable for cell culture, ≥99.0%
别名:
1,3-双[三(羟甲基)甲氨基]丙烷
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关于此项目
线性分子式:
CH2[CH2NHC(CH2OH)3]2
化学文摘社编号:
分子量:
282.33
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
264-899-3
Beilstein/REAXYS Number:
1786109
MDL number:
产品名称
BIS-TRIS丙烷, BioPerformance Certified, suitable for cell culture, ≥99.0%
InChI key
HHKZCCWKTZRCCL-UHFFFAOYSA-N
InChI
1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
SMILES string
OCC(CO)(CO)NCCCNC(CO)(CO)CO
grade
BioPerformance Certified
assay
≥99.0%
form
crystals
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin and bioburden, tested
useful pH range
6.3-9.5
pKa (25 °C)
(1) 6.8, (2) 9.0
mp
164-165 °C (lit.)
application(s)
diagnostic assay manufacturing
foreign activity
DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
Quality Level
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Application
- 中和的嵌合DNA探针改善纳米线场效应晶体管中富含GC RNA的检测。: 在纳米线场效应晶体管的开发过程中，研究人员使用BIS-TRIS丙烷来检测富含GC RNA。该研究证实了缓冲液′在增进这些生物传感器特异性和灵敏度方面的作用 （Chou et al., 2019）。
- 通过优化用于测定重组活化凝血因子VII药物产品中无γ-羧基谷氨酸（Gla）结构域杂质的阴离子交换色谱法来消除鬼峰（ghost peak）: 在优化色谱法的过程中研究人员使用BIS-TRIS丙烷来消除药物杂质分析中的鬼峰。该缓冲液的′有效性确保了药物制剂中杂质准确可靠的检测（Tahmasbi et al., 2019）。
Other Notes
很容易将 BIS-TRIS 丙烷产品规格与 BIS-TRIS 丙烷规格表进行比较。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
E Csuhai et al.
Biochemistry, 34(38), 12411-12419 (1995-09-26)
A secreted dibasic cleaving peptidase capable of converting dynorphins into Leu-enkephalin-Arg6 was purified from the medium of EL-4 mouse thymoma cells. The enzyme is a novel metalloendopeptidase with a neutral pH optimum (6.9) and a molecular weight of approximately 130
S J Smith et al.
The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)
The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)
R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
Michael C Breadmore
Journal of chromatography. A, 1217(24), 3900-3906 (2010-05-11)
An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could
M H Hopkins et al.
Journal of enzyme inhibition, 6(3), 195-199 (1992-01-01)
It is hypothesized that buffers capable of forming a Schiff base with the PLP of gamma-aminobutyric acid aminotransferase (GABA-AT) may lead to denaturation and inactivation of the enzyme. On the basis of this hypothesis three new methods for the selective
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