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Merck
CN

B5002

Sigma-Aldrich

5-溴-2′-脱氧尿苷

≥99% (HPLC)

别名:

5-BrdU, 5-溴-1-(2-脱氧-β-D-呋喃核糖)尿嘧啶, 5-溴脱氧尿苷, BUdR

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About This Item

经验公式(希尔记法):
C9H11BrN2O5
CAS号:
分子量:
307.10
Beilstein:
30395
EC 号:
MDL编号:
UNSPSC代码:
41106305
PubChem化学物质编号:
NACRES:
NA.51

生物来源

synthetic (organic)

质量水平

检测方案

≥99% (HPLC)

形式

powder

mp

191-194 °C (dec.) (lit.)

溶解性

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

储存温度

−20°C

SMILES字符串

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

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应用

5-溴-2′-脱氧尿苷用于:
  • 用作研究电痉挛性癫痫对海马神经发生的影响的补充剂
  • 用作研究斑马鱼成鱼基因丧失功能的补充剂
  • 心脏T淋巴细胞增殖试验{133]

5-溴-2′-脱氧尿苷(5-BrdU)是一种可插入到DNA中的胸苷类似物。 5-BrdU日常被广泛用于对DNA合成进行测量以及对分裂的细胞进行标记。 因此,5-BrdU用于研究细胞信号传导和诱导细胞增殖的其他过程。

生化/生理作用

5-溴-2′-脱氧尿苷(BrdU)用于分析细胞增殖,因为它在细胞周期的S期可轻松掺入DNA。BrdU刺激白血病细胞系中的细胞分化和成熟,同时抑制Friend红白血病细胞分化。随后通过诸如流式细胞分析或荧光显微分析方法研究掺入BrdU时,通常使用带有荧光标记的BrdU特异性抗体来检测掺入的BrdU。
用作遗传研究中诱变剂的胸苷类似物。在 S 期可选择性结合到细胞 DNA 中。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Muta. 1B - Repr. 2

WGK

WGK 2

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

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  1. How does BrdU, Product B5002, work as a Thymidine analog?

    BrdU replaces Thymidine during the S-phase of cell division.

  2. What are the hazards associated with BrdU, Product B5002?

    BrdU is a known carcinogen and caution should be exercised when handling.  Click on the MSDS hyperlink located on the product detail page for Product No. B5002, 5-Bromo-2´-deoxyuridine. 

  3. How long do I treat cells with Product B5002, BrdU to incorporate it into the DNA?

    Actively growing cells may be treated with 10 μm BrdU for 1 hour.

  4. What can I use to detect BrdU, Product B5002 in vivo and in vitro?

    For the immunohistochemical detection of BrdU, we recommend Product No. B2531, Monoclonal Anti-BrdU antibody produced in mouse.

  5. What is the stability of Product B5002, BrdU?

    BrdU is stable as a powder, kept in the freezer and has a recommended re-test date of two years according to the Product Specification Sheet for Product No. B5002.  Please consult our Product Dating Information Statement for more information on re-test dated products.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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