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Merck
CN

B7166

5-溴尿嘧啶核苷 5′-三磷酸盐 钠盐

≥93%, synthetic (organic), powder

别名:

5-BrUTP

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关于此项目

经验公式(希尔记法):
C9H14BrN2O15P3 · xNa+
化学文摘社编号:
分子量:
563.04 (free acid basis)
UNSPSC Code:
41106305
PubChem Substance ID:
NACRES:
NA.51
Assay:
≥93%
Biological source:
synthetic (organic)
Form:
powder
Solubility:
water: 50 mg/mL, clear, colorless
Storage temp.:
−20°C
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产品名称

5-溴尿嘧啶核苷 5′-三磷酸盐 钠盐, ≥93%

SMILES string

OP(OP(OP(O)(O)=O)(O)=O)(OC[C@H]1O[C@@H](N(C=C2Br)C(NC2=O)=O)[C@H](O)[C@@H]1O)=O.[Na]

InChI

1S/3Na/q3*+1

InChI key

BVPYARCRLTXGBD-UHFFFAOYSA-N

biological source

synthetic (organic)

assay

≥93%

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

−20°C

Quality Level

相关类别

General description

5-溴尿嘧啶核苷5′-三磷酸盐(5-BrUTP)用于通过标记核糖核酸(RNA)来测量转录。通过5-BrUTP掺入标记的RNA或细胞可以用抗体进行免疫检测。

Application

5-溴啶5′-三磷酸钠盐已用于:
  • 推断胚胎干细胞(ES)细胞(已分化和未分化)与终分化NIH3T3细胞之间的染色体混合
  • 使用成纤维细胞
  • 和转染MDA-MB-231细胞在体内转录分析中标记新生RNA
  • 标记用于核连缀实验的RNA

5-溴尿嘧啶核苷 5′-三磷酸 (5-BrUTP) 用于通过标记核糖核酸 (RNA) 测量转录。通过 5-BrUTP 掺入标记的 RNA 或细胞可用抗体进行免疫学检测。

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Jose Candelario et al.
Experimental cell research, 317(3), 319-329 (2010-10-27)
Lamin A is a component of the nuclear lamina that plays a major role in the structural organization and function of the nucleus. Lamin A is synthesized as a prelamin A precursor which undergoes four sequential post-translational modifications to generate
Yang Wu et al.
Protein & cell, 1(5), 491-500 (2011-01-05)
Enterovirus 71 (EV71), one of the major causative agents for hand-foot-and-mouth disease (HFMD), has caused more than 100 deaths among Chinese children since March 2008. The EV71 genome encodes an RNAdependent RNA polymerase (RdRp), denoted 3D(pol), which is central for
Shovamayee Maharana et al.
Nucleic acids research, 44(11), 5148-5160 (2016-03-05)
Chromosome territories (CTs) in higher eukaryotes occupy tissue-specific non-random three-dimensional positions in the interphase nucleus. To understand the mechanisms underlying CT organization, we mapped CT position and transcriptional changes in undifferentiated embryonic stem (ES) cells, during early onset of mouse
Claire Tolza et al.
Molecular cancer research : MCR, 17(10), 1999-2014 (2019-07-14)
The architectural chromatin protein HMGA1 and the transcription factor Fra-1 are both overexpressed in aggressive triple-negative breast cancers (TNBC), where they both favor epithelial-to-mesenchymal transition, invasion, and metastasis. We therefore explored the possibility that Fra-1 might be involved in enhanced
Hong-Thuy Bui et al.
Reproduction (Cambridge, England), 141(1), 67-77 (2010-10-27)
Several lines of evidence indicate that the formation of a transcriptionally repressive state during the two-cell stage in the preimplantation mouse embryo is superimposed on the activation of the embryonic genome. However, it is difficult to determine the profile of

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