B7166
5-溴尿嘧啶核苷 5′-三磷酸盐 钠盐
≥93%, synthetic (organic), powder
别名:
5-BrUTP
产品名称
5-溴尿嘧啶核苷 5′-三磷酸盐 钠盐, ≥93%
生物来源
synthetic (organic)
质量水平
方案
≥93%
表单
powder
溶解性
water: 50 mg/mL, clear, colorless
储存温度
−20°C
SMILES字符串
OP(OP(OP(O)(O)=O)(O)=O)(OC[C@H]1O[C@@H](N(C=C2Br)C(NC2=O)=O)[C@H](O)[C@@H]1O)=O.[Na]
InChI
1S/3Na/q3*+1
InChI key
BVPYARCRLTXGBD-UHFFFAOYSA-N
一般描述
5-溴尿嘧啶核苷5′-三磷酸盐(5-BrUTP)用于通过标记核糖核酸(RNA)来测量转录。通过5-BrUTP掺入标记的RNA或细胞可以用抗体进行免疫检测。
应用
5-溴啶5′-三磷酸钠盐已用于:
- 推断胚胎干细胞(ES)细胞(已分化和未分化)与终分化NIH3T3细胞之间的染色体混合
- 使用成纤维细胞
- 和转染MDA-MB-231细胞在体内转录分析中标记新生RNA
- 标记用于核连缀实验的RNA
5-溴尿嘧啶核苷 5′-三磷酸 (5-BrUTP) 用于通过标记核糖核酸 (RNA) 测量转录。通过 5-BrUTP 掺入标记的 RNA 或细胞可用抗体进行免疫学检测。
警示用语:
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Claire Tolza et al.
Molecular cancer research : MCR, 17(10), 1999-2014 (2019-07-14)
The architectural chromatin protein HMGA1 and the transcription factor Fra-1 are both overexpressed in aggressive triple-negative breast cancers (TNBC), where they both favor epithelial-to-mesenchymal transition, invasion, and metastasis. We therefore explored the possibility that Fra-1 might be involved in enhanced
Bianca S Heinrich et al.
PLoS pathogens, 6(6), e1000958-e1000958 (2010-06-30)
Positive-strand and double-strand RNA viruses typically compartmentalize their replication machinery in infected cells. This is thought to shield viral RNA from detection by innate immune sensors and favor RNA synthesis. The picture for the non-segmented negative-strand (NNS) RNA viruses, however
Hong-Thuy Bui et al.
Reproduction (Cambridge, England), 141(1), 67-77 (2010-10-27)
Several lines of evidence indicate that the formation of a transcriptionally repressive state during the two-cell stage in the preimplantation mouse embryo is superimposed on the activation of the embryonic genome. However, it is difficult to determine the profile of
Erin E Duffy et al.
Wiley interdisciplinary reviews. RNA, 10(1), e1513-e1513 (2018-10-30)
Cellular RNA levels are the result of a juggling act between RNA transcription, processing, and degradation. By tuning one or more of these parameters, cells can rapidly alter the available pool of transcripts in response to stimuli. While RNA sequencing
Jose Candelario et al.
Experimental cell research, 317(3), 319-329 (2010-10-27)
Lamin A is a component of the nuclear lamina that plays a major role in the structural organization and function of the nucleus. Lamin A is synthesized as a prelamin A precursor which undergoes four sequential post-translational modifications to generate
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