B7185
Anti-Breast Cancer Resistance Protein antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-BCRP
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC (p), WB
Citations:
5
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~70 kDa
species reactivity
mouse, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using heat-retrieved tissue sections from human term placenta by indirect immunoperoxidase staining of syncytiotrophoblasts., western blot: 2.5-5 μg/mL using whole extract of human term placenta or mouse kidney and a chemiluminescent detection reagent
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ABCG2(9429)
mouse ... Abcg2(26357)
General description
Anti-Breast Cancer Resistance Protein is developed in rabbit using as immunogen a synthetic peptide of human breast cancer resistance protein (BCRP). BCRP, also designated ABCG2, ABCP, placenta specific ATP-binding transporter and mitoxantrone-resistance protein (MXR), is a member of the ABCG subfamily of the large ATP binding cassette (ABC) transporter family of transmembrane proteins. BCRP is a half-transporter, with a single ATP binding cassette and transmembrane region. BCRP is normally expressed in human placental syncytiotrophoblasts, mammary ducts and lobules, endothelial cells and stem cells of different tissues.
Immunogen
synthetic peptide corresponding to amino acid residues 150-167 of human breast cancer resistance protein with C-terminal added cysteine, conjugated to KLH.
Application
Anti-Breast Cancer Resistance Protein antibody produced in rabbit has been used in:
- western blotting
- immunocytochemistry
- immunohistochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Up regulation of breast cancer resistance protein (BCRP) expression may be found in vivo and in vitro in both drugs treated and untreated tumors of different types. Breast cancer resistance protein transports various xenobiotics to the extracellular space through a process energized by ATP hydrolysis. In addition, to its role in protecting cells and tissues against xenobiotics especially in the intestine, liver, placenta and the blood-brain barrier, BCRP appears to be involved in hypoxic defense mechanisms and the protection and regulation of stem cells.
Physical form
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Rare variants in the ABCG2 promoter modulate in vivo activity
Eclov RJ, et al.
Drug Metabolism and Disposition, 46(5), 636-642 (2018)
Camilla Natasha Cederbye et al.
Scientific reports, 6, 26997-26997 (2016-06-04)
Overexpression of the ATP-dependent drug efflux pump ABCG2 is a major molecular mechanism of multidrug resistance in cancer and might be a predictive biomarker for drug response. Contradictory results have been reported for immunohistochemical studies of ABCG2 protein expression in
Arsenic-specific stem cell selection during malignant transformation
Tokar EJ, et al.
Journal of the National Cancer Institute, 638-649 (2010)
Breast cancer resistance protein in pharmacokinetics and drug-drug interactions
Xia CQ, et al.
Expert Opinion on Drug Metabolism & Toxicology, 1(4), 595-611 (2005)
Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 expression and signalling
Nakanishi T, et al.
British Journal of Cancer, 102(5), 815-815 (2010)
商品
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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