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Merck
CN

B9146

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷

≥98%, powder, suitable for identification of lac+ bacterial colonies

别名:

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, BCIG, X-Gal

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关于此项目

经验公式（希尔记法）:
C14H15BrClNO6
化学文摘社编号:
7240-90-6
分子量:
408.63
Beilstein:
1402009
EC 号:
230-640-8
MDL编号:
MFCD00005666
UNSPSC代码:
12352204
PubChem化学物质编号:
57653968
NACRES:
NA.83

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产品名称

5-溴-4-氯-3-吲哚基-β-吡咯-D-半乳糖苷, ≥98%

质量水平

200

方案

≥98%

表单

powder

溶解性

DMF: methanol (1:1): soluble 50 mg/mL

适用性

suitable for identification of lac+ bacterial colonies

储存温度

−20°C

SMILES字符串

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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相关类别

应用

5-溴-4-氯-3-吲哚 β-D-半乳吡喃糖苷已被用于验证Caki-2/LacZ克隆的转导是否成功。它还可用于转化子的蓝白班筛选。
X-Gal是β-半乳糖苷酶的发色底物，可产生丰富的蓝色，与背景相比可以直观地检测到。 X-Gal是lac+基因型重组细菌菌落蓝白斑筛选的首选基质。

制备说明

储备溶液（100 mg/ml）是通过将 X-Gal 溶解在二甲基甲酰胺中制备的，该溶液应避光储存在 −20 °C。无需灭菌。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

涉药品监管产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000503315
0000503315
0000496949
0000496949
0000483117

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