B9304
Anti-Bcl-x antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-BCL2L1
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Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), WB
Citations:
9
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 26-30 kDa
species reactivity
mouse, human, rat
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human colon adenocarcinoma sections, microarray: suitable, western blot: 1:2,000 using human Burkit lymphoma Raji cell lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BCL2L1(598)
mouse ... Bcl2l1(12048)
rat ... Bcl2l1(24888)
General description
Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x, long isoform (Bcl-x) gene is mapped to human chromosome 20q11.21. BCL2L1 belongs to the Bcl-2 family. The bcl-x gene on alternative splicing leads two distinct proteins. It comprises multiple Bcl-2 homology (BH3) domains. Bcl-xL is expressed in the adult brain.(10)
Immunogen
synthetic peptide corresponding to the N-terminal of human Bcl-x (amino acids 2-20) conjugated to BSA. This sequence is identical in human and mouse Bcl-xS, Bcl-xL, Bcl-Xβ and Bcl-Xγ isoforms and is highly conserved in rat Bcl-xS and Bcl-xL (single amino acid substitution).
Application
Anti-Bcl-x antibody produced in rabbit may be used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x arelong isoforms (Bcl-x) of proteins that are involved in regulating apoptosis. Similar to Bcl-2, Bcl-xL can inhibit cell death upon growth factor removal, when transfected in an IL-3 dependent cell line. Bcl-xL can form ion channels in biological membranes and may maintain cell survival by regulating the permeability of intracellular membranes. Bcl-xL may bind to apoptotic protease activating factor 1 (Apaf-1) preventing it from activating caspase 9. Like Bcl-2, Bcl-x activity is regulated by specific phosphorylation of a 60-amino acid regulatory loop region. Bcl-xL has been shown to regulate cell response to oxidative stress, cellular resistance to DNA damage-induced apoptosis by chemotherapeutic agents and ionizing radiation, and favors neurite outgrowth.
The antibody reacts with Bcl-xL.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in "frost-free" freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Megan Stevens et al.
Frontiers in genetics, 10, 804-804 (2019-09-26)
Apoptosis plays a vital role in cell homeostasis during development and disease. Bcl-x, a member of the Bcl-2 family of proteins, is a mitochondrial transmembrane protein that functions to regulate the intrinsic apoptosis pathway. An alternative splicing (AS) event in
A J Minn et al.
Nature, 385(6614), 353-357 (1997-01-23)
Bcl-2-related proteins are critical regulators of cell survival that are localized to the outer mitochondrial, outer nuclear and endoplasmic reticulum membranes. Despite their physiological importance, the biochemical function of Bcl-2-related proteins has remained elusive. The three-dimensional structure of Bcl-xL, an
Marisa A Cubilla et al.
Investigative ophthalmology & visual science, 54(1), 313-322 (2012-12-06)
Glucocorticoids are best known by their protective effect on retinal photoreceptor damage. However, they could also be involved in photoreceptor homeostasis under basal, nonstressful conditions. Therefore, we aimed to study glucocorticoid-induced changes of survival-related molecules in male mice retinas under
Chloe F A Warren et al.
Cell death & disease, 10(3), 177-177 (2019-02-23)
The BCl-2 family has long been identified for its role in apoptosis. Following the initial discovery of BCL-2 in the context of B-cell lymphoma in the 1980s, a number of homologous proteins have since been identified. The members of the
Jianfang Wang et al.
Cellular signalling, 23(12), 2030-2038 (2011-08-16)
Functional analysis of a Bcl-xL phosphorylation mutant series has revealed that cells expressing Bcl-xL(Ser49Ala) mutant are less stable at G2 checkpoint after DNA damage and enter cytokinesis more slowly after microtubule poisoning, than cells expressing wild-type Bcl-xL. These effects of
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