产品名称
Anti-Avidin antibody, Mouse monoclonal, biotin conjugate, clone WC19.10, purified from hybridoma cell culture
biological source
mouse
conjugate
biotin conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
WC19.10, monoclonal
form
buffered aqueous solution
technique(s)
direct ELISA: suitable
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Anti-Avidin-Biotin antibody, Mouse monoclonal has been used for fluorescence in situ hybridization (FISH) analysis of chromosomes X and 8 in lymphocytes isolated from women
Biochem/physiol Actions
Avidin has a high binding affinity for the vitamin, biotin. Biotin conjugated monoclonal anti-avidin increases specific avidin-biotin complex promoting the sensitivity of avidin-biotin immunoassays.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Avidin is a glycoprotein (68 kDa) composed of four identical polypeptides chains isolated from egg whites.Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.
Immunogen
Purified egg white avidin.
Physical form
Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Chapter 11 - (Strept)avidin?Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
Chapter 16 - Biotechnological Approaches for Insect Pest Management
Integrated Pest Management Current Concepts and Ecological Perspective, 311-335 (2014)
Murine monoclonal anti-avidin antibodies enhance the sensitivity of avidin-biotin immunoassays and immunohistologic staining
Cassano WF, et al.
Journal of Immunological Methods, 117(2), 169-174 (1989)
Spontaneous and spindle poison-induced micronuclei and chromosome non-disjunction in cytokinesis-blocked lymphocytes from two age groups of women
Bakou K, et al.
Mutagenesis, 17(3), 233-239 (2002)
Jaeho Kim et al.
Ultrasonography (Seoul, Korea), 41(2), 382-393 (2021-12-29)
The purpose of this study was to evaluate if transcutaneous application of low-intensity ultrasound can locally enhance the effects of finasteride on hair growth in a murine model of androgenic alopecia (AA). AA mice (injected twice per week with testosterone
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