B9673
1-溴-3-氯丙烷
for isolation of RNA
别名:
1-溴-3-氯丙烷, 1,3-溴氯丙烷, I-BCP, 三亚甲基氯溴化合物
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关于此项目
线性分子式:
Cl(CH2)3Br
化学文摘社编号:
分子量:
157.44
UNSPSC Code:
41105515
NACRES:
NA.52
PubChem Substance ID:
EC Number:
203-697-1
Beilstein/REAXYS Number:
605278
MDL number:
Assay:
≥98.5% (GC)
Grade:
Molecular Biology
Bp:
144-145 °C (lit.)
General description
BCP毒性较小，可用于代替氯仿。在TRI试剂单步提取方法中使用BCP，可降低RNA被DNA污染的可能性。它对分离所得RNA的质量或数量无不良影响。1-溴-3-氯丙烷是用于核酸分离方案的相分离试剂。
Application
1-溴-3-氯丙烷已用于分子生物学应用，可从下列物质分离高质量RNA：
- 猫牙齿
- 小鼠大脑和肝脏组织
- 人子宫颈外上皮细胞
- 胎盘样品
- 人间充质基质细胞(hMSC)
Features and Benefits
- 氯仿的低毒性替代品
- 适用于TRI试剂RNA提取法，可降低DNA污染的可能性
Other Notes
BCP毒性较小，可用于代替氯仿。在TRI试剂单步提取方法中使用BCP，可降低RNA被DNA污染的可能性。它对分离所得RNA的质量或数量无不良影响。
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
此项目有
Isolation of total RNA from transgenic mouse melanoma subsets using fluorescence-activated cell sorting
Microarray methods for drug discovery, 27-44 (2010)
Differences in X-chromosome transcriptional activity and cholesterol metabolism between placentae from swine breeds from Asian and Western origins
Bischoff SR, et al.
Testing, 8(1), e55345-e55345 (2013)
Ghalia Boubaker et al.
PLoS neglected tropical diseases, 14(5), e0007640-e0007640 (2020-05-23)
We present a comprehensive analysis of the hepatic miRNA transcriptome at one month post-infection of experimental primary alveolar echinococcosis (AE), a parasitic infection caused upon ingestion of E. multilocularis eggs. Liver tissues were collected from infected and non-infected C57BL/6 mice
Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation.
P Chomczynski et al.
Analytical biochemistry, 225(1), 163-164 (1995-02-10)
Neuroprotection Induced by Energy and Protein-Energy Undernutrition Is Phase-Dependent After Focal Cerebral Ischemia in Mice
de Carvalho TS, et al.
Translational Stroke Research, 1-12 (2019)
实验方案
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
TRI试剂是一种单步RNA分离试剂，可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法，以及各种样品提取方法和故障排除方案。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B9673-200ML | 04061833443897 |
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