制造商/商品名称
(BCK- EdU488FC100)
荧光
λex 496 nm; λem 516 nm
运输
wet ice
储存温度
2-8°C
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一般描述
Baseclick品牌的EdU流式细胞分析试剂盒(EdU Flow Cytometry Kit)为直接检测DNA合成提供了替代BrdU和[3H]胸苷检测的出色方法。EdU(5-乙炔基-2′-脱氧尿苷)是一种胸苷类似物，可在DNA合成活跃期掺入到DNA中。与溴脱氧尿苷(BrdU)检测法不同，EdU流式细胞术法不依赖抗体，因此并不需要进行DNA变性以检测掺入的核苷。恰恰相反，EdU流式细胞试剂盒利用点击化学法来检测不同种类的染料荧光示值。此外，简化的检测方案不仅减少总步骤数，而且大幅缩短总用时。这一简单的点击化学检测程序在30分钟内即可完成，兼容的多重检测可提供内容和含义丰富的结果。
应用
流式细胞术
警示用语：
Danger
危险分类
Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
新产品
此项目有
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
商品
Explore the EdU Cell Proliferation Assay for fast DNA synthesis detection in cell biology, oncology, and drug research.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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