产品名称
ClickTech EdU Cell Proliferation Kit 647 for FC, sufficient for 100 assays
fluorescence
λex 641 nm; λem 663 nm
shipped in
wet ice
storage temp.
2-8°C
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Application
Flow Cytometry
General description
The Baseclick EdU Flow Cytometry Kits provide a superior alternative to BrdU and [3H]thymidine assays for directly measuring DNA synthesis. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU Flow Cytometry Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU Flow Cytometry Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
新产品
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Alexander Lang et al.
Cancers, 12(4) (2020-04-25)
The histone demethylase UTX (gene: KDM6A) directs cell and tissue differentiation during development. Deleterious mutations in KDM6A occur in many human cancers, most frequently in urothelial carcinoma. The consequences of these mutations are poorly understood; plausibly, they may disturb urothelial
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
商品
Explore the EdU Cell Proliferation Assay for fast DNA synthesis detection in cell biology, oncology, and drug research.
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