HDAC Developer (2X)

HDAC assay developer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience′s HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included as an ′inhibitor stop′ for class I and II HDACs.Datasheet

Study of HDAC kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications and cell-based HDAC assays.


Datasheet and Protocols may be found here.

A 2x concentrate. Product is to be used (undiluted) at 1:1 ratio with HDAC reaction.

Avoid freeze/thaw cycles. Use product undiluted.