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BX-0100-30

Human Spinal Motor Neurons

Human iPSC line, Fully differentiated, Healthy Male Control (no known neurological disorders), Cryopreserved

别名:

Lower Motor Neurons

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关于此项目

Biological source:
human (iPSC line)
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biological source

human (iPSC line)

form

frozen liquid

packaging

vial of 1 (contains ≥1 million cells), vial of 1 (contains ≥5 million cells)

technique(s)

cell culture | mammalian: suitable

shipped in

liquid nitrogen

storage temp.

-140 to -196°C

General description

Human iPSC-Derived Spinal Motor Neurons. GFP-expressing neurons also available (BX-0101-30-1M). Required supplements for the final cell differentiation and maturation (sold separately): BX-2100-100uL, BX-2020-100uL, BX-2040-100uL. Located in the spinal cord or at the brain stem within the motor cortex, motor neurons indirectly or directly carry signals to muscles, organs, or glands from the brain. These neurons have one axon with multiple dendrites, and can be classified either as upper motor neurons, which signal between the brain and the spinal cord, or lower motor neurons, which carry signals to muscles from the spinal cord. Related diseases: ALS, SMA, Spinal Cord Injury. Primary neurotransmitter released: Acetylcholine.

Application

Function: Spinal Motor Neurons are adherent and exhibit substantial neurite outgrowth within the first week in culture. Spinal Motor Neurons exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings. Calcein staining demonstrates the elaborate processes of the Spinal Motor Neurons in culture.

Features and Benefits

Marker Expression: Fully differentiated Spinal Motor Neurons have high neuronal purity (>90%) and consist of greater than 70% motor neurons. Labeling with the pan-neuronal marker MAP2 (green) and the motor neuron-specific marker FOXP1 (red) highlight the purity of this neuronal product.

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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实验方案

Protocol Guide: Neuron and Microglia Co-Culture Assay

Discover an expert protocol for seeding and co-culturing iPSC-derived neurons and microglia in monoculture. Explore high purity cells and culture reagents.

Protocol Guide: Motor Neuron Monoculture

Discover an expert protocol for seeding and culturing motor neurons in monoculture. Explore high purity motor neurons culture cells and reagents.

Protocol Guide: Microglia Monoculture

Discover an expert protocol for seeding and culturing microglia in monoculture. Explore high purity microglia and cell culture reagents.

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商品

Protocol Guide: Functional Tri-Culture of iPSC-Derived Human Neurons, Astrocytes, and Microglia

Discover an expert protocol for seeding and tri-culturing iPSC-derived neurons, astrocytes, and microglia in monoculture. Explore high purity cells and culture reagents.

Protocol Guide: Cortical GABAergic Neuron Monoculture

Discover an expert protocol for seeding and culturing cortical GABAergic neurons in monoculture. Explore GABAergic neuron culture cells and reagents.

James C Dodge et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(47), 9137-9147 (2020-10-15)
Amyotrophic lateral sclerosis (ALS) is a fatal neuromuscular disease characterized by motor neuron (MN) death. Lipid dysregulation manifests during disease; however, it is unclear whether lipid homeostasis is adversely affected in the in the spinal cord gray matter (GM), and
Lu-Lin Jiang et al.
The Journal of clinical investigation, 129(8), 3103-3120 (2019-05-22)
Mechanisms underlying motor neuron degeneration in amyotrophic lateral sclerosis (ALS) are yet unclear. Specific deletion of the ER-component membralin in astrocytes manifested postnatal motor defects and lethality in mice, causing the accumulation of extracellular glutamate through reducing the glutamate transporter