form
liquid
contains
0.6-1.0% ethanol as stabilizer
suitability
suitable for nucleic acid purification
application(s)
agriculture
Quality Level
General description
氯仿:异戊醇 24:1混合液是一种用于 DNA 提取和沉淀的有机试剂。
Application
氯仿:异戊醇24:1混合液可用于：
- 提取细菌基因组 DNA、水蚤(Daphnia) DNA 和鲑鱼精子。
- 高质量 DNA 提取的单一 DNA 沉淀步骤。
- 蛋白质提取和 RNA 酶处理。
- 酚氧化，从水溶液中分离酚类物质。
- 从棕色脂肪组织 (BAT)、腹股沟白色脂肪组织 (iWAT) 和附睾脂肪组织 (eWAT) 中分离 RNA。
- 采用改良十六烷基三甲基溴化铵 (CTAB) 方法从凤仙花(Impatiens balsamina)中提取总 RNA 。
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
target_organs
Central nervous system, Liver,Kidney
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
185.0 °F
flash_point_c
85 °C
法规信息
新产品
此项目有
A modified SDS-based DNA extraction method from raw soybean
Xia YC, et al.
Bioscience Reports, 39(2), BSR20182271-BSR20182271 (2019)
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
Healey A, et al.
Plant methods, 10(1), 1-8 (2014)
A modified CTAB-based protocol for total RNA extraction from the medicinal plant Impatiens balsamina (Balsaminaceae) for next-generation sequencing studies.
FOONG LC, et al.
Journal of Separation Science, 46(2) (2017)
Defective insulin-stimulated GLUT4 translocation in brown adipocytes induces systemic glucose homeostasis dysregulation independent of thermogenesis in female mice
Picatoste B, et al.
Molecular Metabolism, 53 (2021)
Harnessing microbially generated power on the seafloor.
Tender LM
Nature Biotechnology, 20(8), 821-825 (2002)
相关内容
DNA和RNA提取是分子生物学中的一种基本方法。高质量、高纯度的核酸在一系列研究和临床应用中发挥着重要的作用。因此，核酸纯化是分子生物学和基因组工作流程中的第一步。
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
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