C0581
Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse
clone CN-B1, ascites fluid
别名:
Anti-CALNB1, Anti-CNB, Anti-CNB1
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关于此项目
Conjugate:
unconjugated
Clone:
CN-B1, monoclonal
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
bovine, rat, human, mouse
Citations:
21
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human myenteric and prostatic ganglia
indirect ELISA: suitable
western blot: 1:3,000 using rat brain extract
indirect ELISA: suitable
western blot: 1:3,000 using rat brain extract
Uniprot accession no.:
产品名称
Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse, clone CN-B1, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
CN-B1, monoclonal
contains
15 mM sodium azide
species reactivity
bovine, rat, human, mouse
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human myenteric and prostatic ganglia
indirect ELISA: suitable
western blot: 1:3,000 using rat brain extract
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PPP3R1(5534), PPP3R2(5535)
rat ... Ppp3r1(29748), Ppp3r2(29749)
Application
Monoclonal Anti-Calcineurin (b-Subunit) antibody produced in mouse has been used in immunohistochemistry, indirect ELISA and western blotting.
Biochem/physiol Actions
Calcineurin calcium -binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. immunoreactivity is detected at significant concentrations only in normal and neoplastic neurons regions of the cytoplasm dendrites, axons and axon terminals. A close correlation has been observed between inhibition of calcineurin by the complexes of cyclosporin A/cyclophilin and tacrolimus(FK506) and FK506 binding proteins (FKBPs) and inhibition of apoptosis. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage, such as an ischemic insult.
Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.
In immunoblotting, the antibody recognizes an epitope located on the β-subunit of calcineurin (18 kDa, also called calcineurin B) and does not cross-react with the α-subunit.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Calcineurin (CaN, heterodimeric enzyme) is a Ca(2+)/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca(2+)-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, α (PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit (calcineurin B) is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.
Monoclonal Anti-Calcineurin (β-subunit) (mouse IgM isotype) is derived from the CN-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain calcineurin preparation. Two different calcineurin B has been reported in rat testis.
Immunogen
bovine brain calcineurin
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Protein phosphatase 2B (PP2B, calcineurin) in Paramecium: partial characterization reveals that two members of the unusually large catalytic subunit family have distinct roles in calcium-dependent processes
Fraga D, et al.
Eukaryotic Cell, 9(7), 1049-1063 (2010)
Neural functions of calcineurin in synaptic plasticity and memory
Baumgartel K and Mansuy IM
Learning & Memory, 19(9), 375-384 (2012)
Substitution in position 3 of cyclosporin A abolishes the cyclophilin-mediated gain-of-function mechanism but not immunosuppression
Baumgrass R, et al.
The Journal of Biological Chemistry, 279(4), 2470-2479 (2004)
Moise Bonheur et al.
Nature neuroscience, 26(7), 1295-1307 (2023-06-13)
Neural activity is modulated over different timescales encompassing subseconds to hours, reflecting changes in external environment, internal state and behavior. Using Drosophila as a model, we developed a rapid and bidirectional reporter that provides a cellular readout of recent neural
Jolene M Kim et al.
Neurochemical research (2018-01-05)
Impaired interactions between Calcineurin (Cn) and (Cu/Zn) superoxide dismutase (SOD1) are suspected to be responsible for the formation of hyperphosphorylated protein aggregation in amyotrophic lateral sclerosis (ALS). Serine (Ser)- enriched phosphorylated TDP-43 protein aggregation appears in the spinal cord of
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