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Merck
CN

C0773

胶原酶 来源于溶组织梭菌

high purity, purified by chromatography, Type VII, ≥4 FALGPA units/mg solid, lyophilized powder, ≥700 CDU/mg solid (CDU = collagen digestion units)

别名:

梭菌肽酶 A

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关于此项目

化学文摘社编号:
9001-12-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
MFCD00130830
EC 号:
3.4.24.3 (BRENDA, IUBMB)
Specific activity:
≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)

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type

Type VII

form

lyophilized powder

specific activity

≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)

mol wt

68-130 kDa

purified by

chromatography

composition

Protein, ≥85% biuret

solubility

TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C

foreign activity

caseinase and clostripain ≤1 units/mg protein

storage temp.

−20°C

Quality Level

200

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Application

胶原酶已用于制备研究晚期糖基化终产物（AGE）的动脉组织。该酶还与其他蛋白酶一起用于人肿瘤、小鼠肾、人脑、肺上皮和许多其他组织的解聚。它在肝脏和肾脏灌注研究、胰腺消化和非实质肝细胞的分离中也是有效的。

Biochem/physiol Actions

从组织中有效释放细胞需要胶原酶和中性蛋白酶的共同作用。胶原酶被4克原子钙（Ca2+）/摩尔的酶活化。培养滤液至少含有7种分子量为68-130kDa的不同蛋白酶。最佳pH范围为6.3-8.8。该酶识别序列 -R-Pro-8-X-Gly-Pro-R-，其中X通常是中性氨基酸。
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。

Physical form

含氯化钙的冻干粉

Preparation Note

储液制备：将0.05-0.1 mg胶原酶溶于1 mL含0.36 mM氯化钙的50 mM TES缓冲液(TESCA)中，pH 7.4(37 °C)。采用冷的缓冲液。
请注意，材料不会完全溶解，会出现少量沉淀。沉淀并不会影响溶液的活性。

Other Notes

一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000511276
0000511275
0000511275
0000511276
0000511277

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实验方案

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

使用FALGPA（N-（3- [2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala）进行的胶原酶酶法检测（EC 3.4.24.3）

在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。

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