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Merck
CN

C0888

Sigma-Aldrich

梭菌蛋白酶 来源于溶组织梭菌

≥20 units/mg solid

别名:

梭菌肽酶B, 蛋白酶 来源于溶组织梭菌

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关于此项目

CAS Number:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
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表单

lyophilized powder

质量水平

比活

≥20 units/mg solid

分子量

15.4 kDa
41.7 kDa

杂质

salt, essentially free

储存温度

2-8°C

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相关类别

应用

梭菌蛋白酶(源于溶组织梭菌)已用作灌注液的蛋白水解酶,检测其对管中血细胞比容(tube hematocrit)的影响。还用于T4噬菌体(RB69 gp43)DNA聚合酶(gp43)的限制性分解。

生化/生理作用

梭菌蛋白酶,来自溶组织梭菌,由两条分子量各为41.7 kDa和15.4 kDa的肽链组成。梭菌蛋白酶对Arg-Xaa肽键具有高限制性的底物特异性。因此,梭菌蛋白酶可服务于蛋白测序。据研究,它也可作为催化剂,用于缩合含Arg-Pro肽键的重要药物肽。

包装

无底玻璃瓶。内含物装在锥底内插管中。

分析说明

本品在使用前,要先溶于含1.0 mM乙酸钙的2.5 mM二硫苏糖醇中活化2-3小时。
纯化制品,基本上不含盐

其他说明

25 °C、pH 7.6,在2.5 mM DTT存在条件下,一个单位每分钟水解1.0μmole BAEE。

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Andrew K Ottens et al.
Journal of neurochemistry, 104(5), 1404-1414 (2007-11-27)
Neurotrauma, as in the case of traumatic brain injury, promotes protease over-activation characterized by the select fragmentation of brain proteins. The resulting polypeptides are indicators of biochemical processes, which can be used to study post-injury dynamics and may also be
Maria Baranyi et al.
The Journal of dairy research, 70(2), 189-197 (2003-06-13)
Acid-precipitated rabbit 'whole casein' was digested by trypsin, chymotrypsin, pepsin, and clostripain to screen for possible peptides with antibacterial properties. The peptide fragments were separated by reversed-phase chromatography. The collected fractions were pooled and their antibacterial properties tested against Escherichia
V Witte et al.
Microbiology (Reading, England), 140 ( Pt 5), 1175-1182 (1994-05-01)
Clostripain-specific antibodies were used to analyse the maturation of clostripain prepro-enzyme and core protein heterologously synthesized in Escherichia coli and Bacillus subtilis. Core protein purified from E. coli cells harbouring plasmid pHM3-23 underwent calcium-dependent, self-triggered maturation. Concomitantly, the inactive form
Chang-Kyu Kim et al.
Journal of biotechnology, 131(3), 346-352 (2007-09-05)
In this study, the clostripain gene was modified and its signal sequence was replaced with that of penicillin G acylase (PGA). The core clostripain protein fused to the PGA signal peptide was also prepared. With regard to the expression of
Synthesis of neo-peptidoglycans: an unexpected activity of proteases.
Nicole Wehofsky et al.
Angewandte Chemie (International ed. in English), 41(15), 2735-2738 (2002-08-31)

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