表单
lyophilized powder
质量水平
比活
≥20 units/mg solid
分子量
15.4 kDa
41.7 kDa
杂质
salt, essentially free
储存温度
2-8°C
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应用
梭菌蛋白酶（源于溶组织梭菌）已用作灌注液的蛋白水解酶，检测其对管中血细胞比容(tube hematocrit)的影响。还用于T4噬菌体(RB69 gp43)DNA聚合酶(gp43)的限制性分解。
生化/生理作用
梭菌蛋白酶，来自溶组织梭菌，由两条分子量各为41.7 kDa和15.4 kDa的肽链组成。梭菌蛋白酶对Arg-Xaa肽键具有高限制性的底物特异性。因此，梭菌蛋白酶可服务于蛋白测序。据研究，它也可作为催化剂，用于缩合含Arg-Pro肽键的重要药物肽。
包装
无底玻璃瓶。内含物装在锥底内插管中。
分析说明
本品在使用前，要先溶于含1.0 mM乙酸钙的2.5 mM二硫苏糖醇中活化2-3小时。
纯化制品，基本上不含盐
其他说明
25 °C、pH 7.6，在2.5 mM DTT存在条件下，一个单位每分钟水解1.0μmole BAEE。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Barney Yoo et al.
Journal of the American Chemical Society, 127(49), 17132-17133 (2005-12-08)
We demonstrate that proteases can catalyze the ligation of peptidomimetic oligomers. The enzyme clostripain was used to facilitate the native ligation of N-substituted glycine oligomers, or peptoids. In addition to mediating the efficient condensation of two peptoid fragments, iterative ligation
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The Journal of biological chemistry, 268(18), 13717-13722 (1993-06-25)
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The activity of Clostridium histolyticum proteinase on synthetic substrates.
J D OLGE et al.
Archives of biochemistry and biophysics, 42(2), 327-336 (1953-02-01)
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Angewandte Chemie (International ed. in English), 41(15), 2735-2738 (2002-08-31)
K Guzik et al.
Cell death and differentiation, 14(1), 171-182 (2006-04-22)
The recognition of phosphatidylserine (PS) on the surface of any apoptotic cell is considered to be a key event for its clearance. We challenge this concept by showing that pretreatment of neutrophils with either host or bacterial protease affects their
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