C0897
柠檬酸裂解酶 来源于肺炎克雷伯氏杆菌
lyophilized powder, ≥0.20 unit/mg solid
别名:
柠檬酸草酰乙酸裂解酶
应用
从肺炎克雷伯氏菌中分离的柠檬酸裂解酶可用于消化低分子量人乳组分（>5 kDa，简称为 5kF）。
生化/生理作用
柠檬酸裂解酶存在于若干微生物中，用于催化柠檬酸盐裂解的第一步，形成草乙酸和乙酸盐。该酶含有 3 个多肽亚基，α-亚基（转移酶）、β-亚基（酰基裂解酶）和 γ-亚基（酰基-载体蛋白）。
外形
冻干品，含有牛血清白蛋白、蔗糖和 MgSO4
其他说明
在 pH 7.6，25℃ 条件下，一个单位每分钟将 1.0 μmole 柠檬酸盐转化为草乙酸。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Víctor S Blancato et al.
Journal of bacteriology, 190(22), 7419-7430 (2008-09-23)
The genome of the gram-positive bacterium Enterococcus faecalis contains the genes that encode the citrate lyase complex. This complex splits citrate into oxaloacetate and acetate and is involved in all the known anaerobic bacterial citrate fermentation pathways. Although citrate fermentation
Djamel Drider et al.
Genetics and molecular research : GMR, 3(2), 273-281 (2004-07-22)
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert
M Hupperich et al.
European journal of biochemistry, 192(1), 161-166 (1990-08-28)
The primary structure of the beta-subunit (acyl lyase subunit) of citrate lyase from Klebsiella pneumoniae (ATCC 13,882) was determined with protein chemical methods. The polypeptide chain consists of 289 amino acid residues and has a molecular mass of 31,352 Da.
Loïc Rivière et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(31), 12694-12699 (2009-07-25)
Acetyl-CoA produced in mitochondria from carbohydrate or amino acid catabolism needs to reach the cytosol to initiate de novo synthesis of fatty acids. All eukaryotes analyzed so far use a citrate/malate shuttle to transfer acetyl group equivalents from the mitochondrial
M Martín et al.
FEMS microbiology letters, 174(2), 231-238 (1999-05-26)
The citMCDEFGRP cluster from Leuconostoc paramesenteroides involved in citrate utilization was cloned and its nucleotide sequence determined. Homology of the inferred gene products with characterized enzymes reveals that citP encodes the citrate permease P, citC the citrate ligase and citDEF
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