C-12216
Human Dermal Lymphatic Endothelial Cells (HDLEC)
Juvenile foreskin, 500,000 cryopreserved cells
别名:
HDLEC cells
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关于此项目
NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human foreskin (juvenile dermis)
Morphology:
( endothelial)
biological source
human foreskin (juvenile dermis)
product line
PromoCell®
packaging
pkg of 500,000 cells
morphology
( endothelial)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
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Application
Primary Human Dermal Lymphatic Endothelial Cells (HDLEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin from a single donor and are provided in a cryopreserved format. (Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.)The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: >95% of the cells are CD31 positive and podoplanin positive. In addition, the cells stain positive for Prox-1.Lymphatic vessels transport excess fluids from tissues to the circulatory system and are a major component of the immune system. The transported fluid is nearly cell free. The vessels are highly permeable, because they lack a continuous basal membrane. Lymphatic endothelial cells are involved in pathological alterations of the lymphatic system. During tumor lymphangiogenesis, the lymphatic endothelial cells build new vessels that infiltrate tumors, attract tumor cells, and induce tumor cell metastasis.
Biochem/physiol Actions
Skin
Preparation Note
Subculture Routine –Click here for more information.
Analysis Note
Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Podoplanin positive, CD31 positiveGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link
Legal Information
PromoCell is a registered trademark of PromoCell GmbH
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
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存储类别
12 - Non Combustible Liquids
WGK
WGK 1
闪点 (F)
Not applicable
闪点 (°C)
Not applicable