Human Tracheal Smooth Muscle Cells (HTSMC)

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Primary Human Tracheal Smooth Muscle Cells (HTSMC) are isolated from the trachea from single donors and are provided in a cryopreserved format. HTSMC stain positive for smooth muscle α-actin. They connect the endings of the tracheal cartilage by a contractile bridge called the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the trachea walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, HTSMC are suitable for in vitro studies of asthma and other pulmonary diseases.

Trachea

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Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.