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Merck
CN

C-12720

Human Osteoblasts (HOB)

500,000 cryopreserved cells

别名:

HOB cells

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NACRES:
NA.81
UNSPSC Code:
41106514

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产品名称

Human Osteoblasts (HOB), 500,000 cryopreserved cells

biological source

human bone (knee and hip joints)

packaging

pkg of 500,000 cells

morphology

(osteoblasts)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Analysis Note

Rigid quality control tests are performed for each lot of Human Osteoblasts. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, histochemical tests for alkaline phosphatase and bone mineralization are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions lacking antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Application

Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request) and are provided in a cryopreserved format. They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.Osteoblasts are of mesenchymal origin and are mainly responsible for bone formation and remodeling. In vivo, they produce a collagen type I rich extracellular matrix called the osteoid. During their development, osteoblasts undergo a maturation process called osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.

Biochem/physiol Actions

Bone Tissue

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positive, Mineralization testedGuaranteed population doublings: >10
Recommended Primary Cell Culture Media:Link

Preparation Note

Click here for more information.

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

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Introduction to Primary Cell Culture

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Primary Human Osteoblasts and Osteoblast Growth/Differentiation Media

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Primary Cell Culture Basics

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

原代细胞培养基础知识

什么是原代细胞？关于动物和人类原代细胞系和培养基的全面概述，以及它们如何应用于生物医学细胞培养应用。相对于细胞系，原代培养物的均一性较低。

相关内容

Differentiation of Primary Human Osteoblasts

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

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