Human Osteoblasts (HOB)

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Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request) and are provided in a cryopreserved format. They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.Osteoblasts are of mesenchymal origin and are mainly responsible for bone formation and remodeling. In vivo, they produce a collagen type I rich extracellular matrix called the osteoid. During their development, osteoblasts undergo a maturation process called osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.

Bone Tissue

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Rigid quality control tests are performed for each lot of Human Osteoblasts. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, histochemical tests for alkaline phosphatase and bone mineralization are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions lacking antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: Alkaline phosphatase positive, Mineralization testedGuaranteed population doublings: >10

Recommended Primary Cell Culture Media:Link

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.