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Merck
CN

C1403

Sigma-Aldrich

胆固醇酯酶 来源于假单胞菌

lyophilized powder, ≥200,000 units/g protein

别名:

胆固醇酯酶 来源于荧光假单胞菌, 甾醇酯酰水解酶

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关于此项目

CAS Number:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
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表单

lyophilized powder

质量水平

比活

≥200,000 units/g protein

分子量

~300 kDa

组成

protein, ≥40% biuret

储存温度

−20°C

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一般描述

稳定性:在–20°C稳定至少一年
等电点:5.9 ± 0.1
米氏常数:5.4 x 10‾5M(亚油酸脂),6.6 x 10‾5M(油酸)
3.7 x 10‾5M(亚油酸脂),1.5 x 10‾4M(棕榈酸)
1.2 x 10‾4M(肉豆蔻),2.3 x 10‾5M(硬脂酸酯)
抑制剂:Hg++、Ag+、离子型去垢剂
最适pH值: 7.0 − 9.0
最适温度:40°C
pH稳定性: pH 5.0 − 9.0 (25°C, 24hr)
热稳定性:低于55°C(pH 7.5,10分钟)

应用

当在临床分析中与胆固醇氧化酶结合使用时,该酶可用于酶法测定总胆固醇。

生化/生理作用

胆固醇酯酶(CE)是一种可逆酶,可以水解或合成胆固醇和其他固醇的脂肪酸酯。水不溶性长链脂肪酸酯的水解需要经过胆盐活化。短链脂肪酸和溶血磷脂的水溶性酯的水解则不需要胆盐的活化。它还可水解三、二和单酰基甘油、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中可能具有多种功能。

其他说明

在牛磺胆酸盐存在下,一个单位在 pH7.0,37°C 下每分钟将 1.0μmole胆固醇油酸酯水解成胆固醇和油酸。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Experimental & molecular medicine, 44(10), 603-614 (2012-08-04)
Higher levels of body fat are associated with an increased risk for development numerous adverse health conditions. FTY720 is an immune modulator and a synthetic analogue of sphingosine 1-phosphate (S1P), activated S1P receptors and is effective in experimental models of
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商品

胆固醇酯化可改善运输。胆固醇酯可更轻松地封装在脂蛋白内部-增加了在血液中可轻松运输的胆固醇数量。

Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.

实验方案

Protocol for Assay Procedure for Cholesterol Esterase

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