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Form:
solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (histidine-tagged)
Mol wt:
16 kDa
biological source
human
recombinant
expressed in E. coli (histidine-tagged)
assay
≥90% (SDS-PAGE)
form
solution
mol wt
16 kDa
technique(s)
inhibition assay: suitable
suitability
suitable for molecular biology
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... CYB5A(1528)
General description
细胞色素 b5 (CYB5A) 基因编码细胞色素 b5 蛋白。位于人类 18 号染色体上。在睾丸的 Leydig 细胞和卵巢的卵泡膜细胞中共表达。
Biochem/physiol Actions
细胞色素 b5 (CYB5A) 通过细胞色素 P450 酶介导 NADH 依赖性高铁血红蛋白(红细胞)还原、电子转移至脂肪酸去饱和酶(主要是肝脏)以及类固醇和外源性物质代谢。对雄烯二酮合成至关重要。CYB5A 有助于性腺雄激素的有效合成。
细胞色素b5 是一种膜结合的含血红素的蛋白质,可增强某些P450同工型的催化效率。 其作用机理未知。
膜结合的含血红素的蛋白质;提高某些P450异构体的催化效率。
Physical form
溶于 20 mM 磷酸盐缓冲液 (pH 7.25) 中,含 0.1 mM EDTA
Analysis Note
使用差光谱法测量血红素含量。 对于490nm和424nm之间的吸光度差,使用100mMof-1的消光系数来计算细胞色素b5的浓度。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Melissa R Pitman et al.
Scientific reports, 12(1), 454-454 (2022-01-12)
Sphingosine 1-phosphate (S1P) is a signaling lipid that has broad roles, working either intracellularly through various protein targets, or extracellularly via a family of five G-protein coupled receptors. Agents that selectively and specifically target each of the S1P receptors have
Human adrenal cells that express both beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production
Nakamura Y, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 123(3-5), 122-126 (2011)
CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women
Stark K, et al.
Arthritis Research & Therapy, 17(1), 56-56 (2015)
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