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Form:
solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (histidine-tagged)
Mol wt:
16 kDa
产品名称
细胞色素 b 5 人, recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli (histidine-tagged)
assay
≥90% (SDS-PAGE)
form
solution
mol wt
16 kDa
technique(s)
inhibition assay: suitable
suitability
suitable for molecular biology
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... CYB5A(1528)
Analysis Note
使用差光谱法测量血红素含量。 对于490nm和424nm之间的吸光度差,使用100mMof-1的消光系数来计算细胞色素b5的浓度。
Biochem/physiol Actions
细胞色素 b5 (CYB5A) 通过细胞色素 P450 酶介导 NADH 依赖性高铁血红蛋白(红细胞)还原、电子转移至脂肪酸去饱和酶(主要是肝脏)以及类固醇和外源性物质代谢。对雄烯二酮合成至关重要。CYB5A 有助于性腺雄激素的有效合成。
细胞色素b5 是一种膜结合的含血红素的蛋白质,可增强某些P450同工型的催化效率。 其作用机理未知。
膜结合的含血红素的蛋白质;提高某些P450异构体的催化效率。
General description
细胞色素 b5 (CYB5A) 基因编码细胞色素 b5 蛋白。位于人类 18 号染色体上。在睾丸的 Leydig 细胞和卵巢的卵泡膜细胞中共表达。
Physical form
溶于 20 mM 磷酸盐缓冲液 (pH 7.25) 中,含 0.1 mM EDTA
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Melissa R Pitman et al.
Scientific reports, 12(1), 454-454 (2022-01-12)
Sphingosine 1-phosphate (S1P) is a signaling lipid that has broad roles, working either intracellularly through various protein targets, or extracellularly via a family of five G-protein coupled receptors. Agents that selectively and specifically target each of the S1P receptors have
CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women
Stark K, et al.
Arthritis Research & Therapy, 17(1), 56-56 (2015)
Human adrenal cells that express both beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production
Nakamura Y, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 123(3-5), 122-126 (2011)
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