C2409
肌酸酶 来源于放线杆菌 属
lyophilized powder, ≥4 units/mg solid
别名:
肌酸脒基水解酶
产品名称
肌酸酶 来源于放线杆菌 属, lyophilized powder, ≥4 units/mg solid
biological source
bacterial (Actinobacillus spp.)
form
lyophilized powder
specific activity
≥4 units/mg solid
mol wt
~100 kDa
storage temp.
−20°C
Quality Level
Other Notes
在pH 7.5、37℃条件下，1个单位将每分钟水解1.0 μmol肌酸形成尿素和肌氨酸。
Physical form
含有糖和EDTA 作为稳定剂的冻干粉
Application
肌酸酶与肌氨酸氧化酶混合可用于测定不同pH值、温度、酶比和缓冲液浓度下的肌酸水平。也可以通过使用离心分析仪来测定血浆肌酐水平。
Biochem/physiol Actions
肌酸酶加速肌酸和水分子转化形成肌氨酸和尿素的反应。它总是以同型二聚体状态起作用，并由氯化胆碱诱导。
General description
等电点： 4.6 ± 0.1
米氏常数： 1.9 x 10‾2M（肌酸）
结构： 每摩尔酶2个亚基
抑制剂： Cu++，Hg++，Ag+
最佳pH： 8.0
最佳温度： 40℃
pH稳定性： pH 5.5 − 9.0（25℃，16hr）
热稳定性： 低于50℃（pH 7.5，30分钟）
米氏常数： 1.9 x 10‾2M（肌酸）
结构： 每摩尔酶2个亚基
抑制剂： Cu++，Hg++，Ag+
最佳pH： 8.0
最佳温度： 40℃
pH稳定性： pH 5.5 − 9.0（25℃，16hr）
热稳定性： 低于50℃（pH 7.5，30分钟）
肌酸酶是催化肌酸水解的同型二聚体。它由两个单体亚基和两个指定结构域组成；N和C末端结构域。C端折叠在两个结构相似的结构域内，同时具有α螺旋和反平行β折叠。在这两个结构域之间，一个巯基作为活性位点，其活性无金属依赖性。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Balasundaram Padmanabhan et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 8), 1322-1328 (2002-07-24)
The crystal structure of Actinobacillus creatine amidinohydrolase has been solved by molecular replacement. The amino-acid sequence has been derived from the crystal structure. Crystals belong to space group I222, with unit-cell parameters a = 111.26 (3), b = 113.62 (4)
H Crocker et al.
Journal of clinical pathology, 41(5), 576-581 (1988-05-01)
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages
J F Bazan et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(7), 2473-2477 (1994-03-29)
Amino acid sequence comparison suggests that the structure of Escherichia coli methionine aminopeptidase (EC 3.4.11.18) and the C-terminal domain of Pseudomonas putida creatinase (EC 3.5.3.3) are related. A detailed comparison of the three-dimensional folds of the two enzymes confirms this
Pinar Esra Erden et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 34(2), 223-239 (2006-03-16)
A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and
N A Siddiqi et al.
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia, 17(2), 203-207 (2006-08-15)
Polyoma virus nephropathy (BK virus) is being recognized as an important cause of graft failure. It is usually confused with acute rejection. No cases have been reported from the kingdom of Saudi Arabia. We report a case of a Saudi
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