C2409
肌酸酶 来源于放线杆菌 属
lyophilized powder, ≥4 units/mg solid
别名:
肌酸脒基水解酶
产品名称
肌酸酶 来源于放线杆菌 属, lyophilized powder, ≥4 units/mg solid
biological source
bacterial (Actinobacillus spp.)
form
lyophilized powder
specific activity
≥4 units/mg solid
mol wt
~100 kDa
storage temp.
−20°C
Quality Level
Other Notes
在pH 7.5、37℃条件下，1个单位将每分钟水解1.0 μmol肌酸形成尿素和肌氨酸。
Physical form
含有糖和EDTA 作为稳定剂的冻干粉
Application
肌酸酶与肌氨酸氧化酶混合可用于测定不同pH值、温度、酶比和缓冲液浓度下的肌酸水平。也可以通过使用离心分析仪来测定血浆肌酐水平。
Biochem/physiol Actions
肌酸酶加速肌酸和水分子转化形成肌氨酸和尿素的反应。它总是以同型二聚体状态起作用，并由氯化胆碱诱导。
General description
等电点： 4.6 ± 0.1
米氏常数： 1.9 x 10‾2M（肌酸）
结构： 每摩尔酶2个亚基
抑制剂： Cu++，Hg++，Ag+
最佳pH： 8.0
最佳温度： 40℃
pH稳定性： pH 5.5 − 9.0（25℃，16hr）
热稳定性： 低于50℃（pH 7.5，30分钟）
米氏常数： 1.9 x 10‾2M（肌酸）
结构： 每摩尔酶2个亚基
抑制剂： Cu++，Hg++，Ag+
最佳pH： 8.0
最佳温度： 40℃
pH稳定性： pH 5.5 − 9.0（25℃，16hr）
热稳定性： 低于50℃（pH 7.5，30分钟）
肌酸酶是催化肌酸水解的同型二聚体。它由两个单体亚基和两个指定结构域组成；N和C末端结构域。C端折叠在两个结构相似的结构域内，同时具有α螺旋和反平行β折叠。在这两个结构域之间，一个巯基作为活性位点，其活性无金属依赖性。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Balasundaram Padmanabhan et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 8), 1322-1328 (2002-07-24)
The crystal structure of Actinobacillus creatine amidinohydrolase has been solved by molecular replacement. The amino-acid sequence has been derived from the crystal structure. Crystals belong to space group I222, with unit-cell parameters a = 111.26 (3), b = 113.62 (4)
H Crocker et al.
Journal of clinical pathology, 41(5), 576-581 (1988-05-01)
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages
J F Bazan et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(7), 2473-2477 (1994-03-29)
Amino acid sequence comparison suggests that the structure of Escherichia coli methionine aminopeptidase (EC 3.4.11.18) and the C-terminal domain of Pseudomonas putida creatinase (EC 3.5.3.3) are related. A detailed comparison of the three-dimensional folds of the two enzymes confirms this
Jason A Berberich et al.
Acta biomaterialia, 1(2), 183-191 (2006-05-17)
The enzyme creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine. Continuous use biosensors are becoming more important in the clinical setting; however, long-use creatinine biosensors have not been commercialized due to
Pinar Esra Erden et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 34(2), 223-239 (2006-03-16)
A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and
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