C2506
细胞色素 C 来源于马心脏
≥95% (SDS-PAGE)
别名:
Cytochrome c 来源于马心脏
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关于此项目
Form:
powder
Assay:
≥95% (SDS-PAGE)
Biological source:
horse heart
Mol wt:
12,384
biological source
horse heart
Quality Level
assay
≥95% (SDS-PAGE)
form
powder
mol wt
12,384
technique(s)
cell based assay: suitable
solubility
H2O: soluble 10 mg/mL
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
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Application
细胞色素c已用于：
- 还原由黄嘌呤氧化酶生成的超氧自由基-铁氧化铬c
- 采用MALDI质谱法，用单液滴沉积法进行分析
- 作为标准细胞色素c还原酶活性测定的末端电子受体
- 细胞色素c氧化酶组织化学
- 用于细胞色素C氧化酶活性的测定
- 作为磷酸盐缓冲液的组分，用于采用蓝色天然凝胶电泳 (BN-PAGE) 检测和评估复合物 IV 活性
- 用于检测离体肾脏吞噬细胞中的细胞外超氧阴离子 (ECSA)
马的细胞色素c经过H2O2和UV 处理后的特异性位点和氧化程度已经过表征。研究表明这些位点可能可用作细胞内氧化环境的生物标记。
Biochem/physiol Actions
细胞色素 c 从线粒体外层释放到胞质溶胶中，有助于激活 caspase-9 并启动凋亡蛋白酶级联反应。其与凋亡蛋白酶激活因子-1 (APAF-1) 和三磷酸脱氧腺苷 (dATP) 的相互作用可促进这一过程。(3)各种研究表明，测定肿瘤患者血清中的细胞色素 C 水平可以被认为是体内细胞死亡的潜在标志。
细胞色素c的主要身份是电子载体线粒体蛋白。在细胞内，细胞色素c能够在二价铁和三价铁之间转化，使得它称为了有效的生物电子载体，在动植物细胞内氧化中起到很重要的作用。它通常被视作呼吸作用催化剂，在呼吸底物和氧气之间形成必需的电子桥
Preparation Note
Prepared using TCA.
本品溶于水中(10 mg/mL)应呈深红色至红褐色。
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
B C Hill
The Journal of biological chemistry, 266(4), 2219-2226 (1991-02-05)
The reaction of the electrostatic cytochrome c-cytochrome oxidase complex with oxygen is measured by transient absorption spectroscopy. The oxygen reaction is initiated by photolytic removal of CO from cytochrome oxidase, using a flash-pumped dye laser. The subsequent reaction of the
Vittorio Pandini et al.
The Journal of biological chemistry, 277(50), 48463-48471 (2002-10-09)
Toxoplasma gondii possesses an apicoplast-localized, plant-type ferredoxin-NADP(+) reductase. We have cloned a [2Fe-2S] ferredoxin from the same parasite to investigate the interplay of the two redox proteins. A detailed characterization of the two purified recombinant proteins, particularly as to their
Enzyme-catalyzed free radical reactions with nicotinamide adenine nucleotides. II. Lactate dehydrogenase-catalyzed oxidation of reduced nicotinamide adenine dinucleotide by superoxide radicals generated by xanthine oxidase.
P C Chan et al.
The Journal of biological chemistry, 249(4), 1317-1319 (1974-02-25)
A novel role of the mitochondrial permeability transition pore in (-)-gossypol-induced mitochondrial dysfunction
Warnsmann V, et al.
Mechanisms of Ageing and Development, 170, 45-58 (2018)
?-1,3 glucan derived from Euglena gracilis and Algamune? enhances innate immune responses of red drum (Sciaenops ocellatus L.)
Yamamoto FY, et al.
Fish & Shellfish Immunology, 77, 273-279 (2018)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C2506-50MG | 04061835555888 |
| C2506-1G | 04061835555864 |
| C2506-2G | 04061835521685 |
| C2506-250MG | 04061835543243 |
| C2506-100MG | 04061835555857 |
| C2506-500MG | 04061835555871 |
| C2506-5G | 04061835515868 |
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