C2522
碳酸酐酶同工酶 II 来源于牛红细胞
lyophilized powder, ≥3,000 W-A units/mg protein
别名:
碳酸水解酶, 碳酸盐脱水酶, 碳酸酐酶 II
应用
Sigma 公司的碳酸酐酶已被用于通过测量结合亲和力和化学计量学来分析酶的热力学稳定性。在大鼠自身免疫性胰腺炎研究期间，还使用该酶生成了对碳酸酐酶特异性的 CD 4 + T 细胞系。
生化/生理作用
碳酸酐酶是一种锌金属酶，分子量约为 30,000 Da。这种酶催化二氧化碳水合生成碳酸。参与 pH 和 CO 2 动态平衡、碳酸氢盐和 CO 2 的转运、生物合成反应、骨吸收、钙化、致瘤等许多生理和病理过程。因此，阻断该酶的抑制剂可能对青光眼、癫痫和帕金森氏病具有治疗意义。
其他说明
一个 Wilbur-Anderson (W-A) 单位每分钟将导致 0.02 MTrizma 缓冲液的 pH 值在 0°C 下从 8.3 降至 6.3（一个 W-A 单位基本上相当于一个 Roughton-Booth 单位）。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Arne Schön et al.
Analytical biochemistry, 488, 45-50 (2015-08-05)
Protein aggregation is a major issue affecting the long-term stability of protein preparations. Proteins exist in equilibrium between the native and denatured or partially denatured conformations. Often denatured or partially denatured conformations are prone to aggregate because they expose to
Rui Hou et al.
ACS omega, 8(21), 19057-19071 (2023-06-05)
DNA-encoded library (DEL) is a powerful ligand discovery technology that has been widely adopted in the pharmaceutical industry. DEL selections are typically performed with a purified protein target immobilized on a matrix or in solution phase. Recently, DELs have also
Gérard Liger-Belair et al.
ACS omega, 8(25), 22844-22853 (2023-07-03)
When it comes to champagne tasting, dissolved CO2 is a key compound responsible for the very much sought-after effervescence in glasses. Nevertheless, the slow decrease of dissolved CO2 during prolonged aging of the most prestigious cuvees raises the issue of
Amelia Pollard et al.
Aging, 8(10), 2425-2436 (2016-10-16)
Carbonic anhydrase inhibitors are used to treat glaucoma and cancers. Carbonic anhydrases perform a crucial role in the conversion of carbon dioxide and water into bicarbonate and protons. However, there is little information about carbonic anhydrase isoforms during the process
Hajime Shigemitsu et al.
Nature communications, 11(1), 3859-3859 (2020-08-02)
Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases. Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies. Here, we describe a
实验方案
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
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