form
liquid
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
General description
Mononuclear Cell Medium (Ready-to-use)
Application
Mononuclear Cell Medium is a very rich short-term maintenance medium, which provides utmost cell viability for Mononuclear Cells from umbilical cord blood, adult peripheral blood and other sources, e.g. bone marrow. It is suitable to maintain Mononuclear Cells for up to 48 hours before proceeding with your experiments.Recommended for:Human Mononuclear Cells from Cord Blood (hMNC-CB)Human Mononuclear Cells from Peripheral Blood (hMNC-PB)
Preparation Note
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Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C. Note: The SupplementMix is delivered thawed and can be frozen after arrival without losing any activity
The Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium.
Analysis Note
All lots of PromoCell Mononuclear Cell Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from cord blood and adult peripheral blood. Each lot of PromoCell Mononuclear Cell Medium is checked for unaltered shortterm maintenance of highly viable mononuclear cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
存储类别
10 - Combustible liquids
法规信息
新产品
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商品
Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.
Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
实验方案
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
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