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Merck
CN

C2888

Sigma-Aldrich

乙酰胆碱酯酶 来源于电鳗

Type V-S, lyophilized powder, ≥1,000 units/mg protein

别名:

AChE, 乙酰胆碱乙酰水解酶, 胆碱酯酶，乙酰基

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关于此项目

CAS Number:
9000-81-1
EC 号:
3.1.1.7 (BRENDA, IUBMB)
EC 号:
232-559-3
MDL编号:
MFCD00081268
UNSPSC代码:
12352204
NACRES:
NA.32

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生物来源

Electrophorus electricus

质量水平

200

类型

Type V-S

表单

lyophilized powder

比活

≥1,000 units/mg protein

分子量

280 kDa

组成

Protein, ≥45% biuret

pI 

~5.3

溶解性

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

储存温度

−20°C

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一般描述

分子量： 280 kDa
等电点： 5.5
消光系数： E1%= 18.0（280 nm）

来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体，每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。

应用

电鳗乙酰胆碱酯酶已用于：
  • 乙酰胆碱酯酶活性检测
  • 研究其对爪蟾胚胎海马体细胞的影响
  • 研究乙酰胆碱抑制剂对突触发育和可塑性的影响

生化/生理作用

体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。
乙酰胆碱酯酶(AChE)的活性表现于其可降解神经肌肉接头和胆碱能神经元突触间隙处的乙酰胆碱。该酶参与一氧化氮信号通路。

外形

含 Tris 缓冲盐的冻干粉

分析说明

使用乙酰胆碱作为底物获得的活性是用丁基胆碱获得的活性的 30-100 倍，使用来自电鳗的乙酰胆碱酯酶。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
一个单元将在 pH8.0，37℃下，每分钟将 1.0 μmole 乙酰胆碱水解成胆碱和乙酸盐。

免责声明

对于稀释溶液（<1 mg/ml），加入 1mg/ml BSA 以稳定。在稀释的中性磷酸盐缓冲液中含有至少 1mg 蛋白质/ml 的溶液在冷藏至少 6 个月后是稳定的。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000472955
0000472954
0000472954
0000472955
0000450595

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are solutions of Product C2888, Acetylcholinesterase from Electrophorus electricus (electric eel), harmed by freezing?

    Yes, solutions may be harmed by freezing. The data sheet for Product C2888 indicates that solutions stabilized by the addition of BSA are stable when kept refrigerated for up to six months. A customer has reported to us that they tried freezing a solution to which BSA was added; they found that the solution had a 40 percent loss of activity after four months in the freezer. So our best recommendation is that solutions be stabilized with BSA and kept refrigerated, not frozen.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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