跳转至内容
Merck
CN

C2900

氯霉素乙酰转移酶 来源于大肠杆菌

lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

别名:

CAT, 乙酰辅酶 A:氯霉素 3-O-乙酰转移酶

登录 查看组织和合同定价。

选择尺寸


关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

氯霉素乙酰转移酶 来源于大肠杆菌, lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

form

lyophilized powder

specific activity

50,000-150,000 units/mg protein (Lowry)

mol wt

75 kDa (three identical subunits)

shipped in

dry ice

storage temp.

−20°C

Quality Level

Gene Information

Escherichia coli ... cat(2847485)

正在寻找类似产品? 访问 产品对比指南

Biochem/physiol Actions

这种酶是细菌对氯霉素产生抗性的原因。催化乙酰辅酶 A 和氯霉素转化为辅酶 A 和氯霉素 3-乙酸酯。

Other Notes

在 pH 7.8 和 25°C 下,一单位本品每分钟可以将 1.0 纳摩尔氯霉素和乙酰辅酶 A 转化为氯霉素 3-乙酸酯和辅酶 A。

Physical form

部分纯化;含 Tris 缓冲盐。

General description

The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Hirofumi Nariya et al.
Applied and environmental microbiology, 77(23), 8439-8441 (2011-10-04)
A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo
Tapan Biswas et al.
Protein science : a publication of the Protein Society, 21(4), 520-530 (2012-02-02)
Novel antibiotics are needed to overcome the challenge of continually evolving bacterial resistance. This has led to a renewed interest in mechanistic studies of once popular antibiotics like chloramphenicol (CAM). Chloramphenicol acetyltransferases (CATs) are enzymes that covalently modify CAM, rendering
Vanessa Fuentes et al.
Eukaryotic cell, 11(6), 725-734 (2012-04-03)
Synthesis of functional mRNA in eukaryotes involves processing of precursor transcripts, including the addition of a poly(A) tail at the 3' end. A multiprotein complex recognizes a polyadenylation signal, generally the hexanucleotide AAUAAA in metazoans, to direct processing of the
Tuija A A Pöyry et al.
Journal of virology, 85(19), 10178-10188 (2011-08-05)
Translation initiation dependent on the foot-and-mouth disease virus (FMDV) internal ribosome entry site (IRES) occurs at two sites (Lab and Lb), 84 nucleotides (nt) apart. In vitro translation of an mRNA comprising the IRES and Lab-Lb intervening segment fused to
Ziyaad Valley-Omar et al.
Virology journal, 8, 462-462 (2011-10-07)
HIV-1 Gag virus like particles (VLPs) used as candidate vaccines are regarded as inert particles as they contain no replicative nucleic acid, although they do encapsidate cellular RNAs. During HIV-1 Gag VLP production in baculovirus-based expression systems, VLPs incorporate the

实验方案

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持