C2900
氯霉素乙酰转移酶 来源于大肠杆菌
lyophilized powder, 50,000-150,000 units/mg protein (Lowry)
别名:
CAT, 乙酰辅酶 A：氯霉素 3-O-乙酰转移酶
表单
lyophilized powder
质量水平
比活
50,000-150,000 units/mg protein (Lowry)
分子量
75 kDa (three identical subunits)
运输
dry ice
储存温度
−20°C
基因信息
Escherichia coli ... cat(2847485)
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一般描述
The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.
生化/生理作用
这种酶是细菌对氯霉素产生抗性的原因。催化乙酰辅酶 A 和氯霉素转化为辅酶 A 和氯霉素 3-乙酸酯。
外形
部分纯化；含 Tris 缓冲盐。
其他说明
在 pH 7.8 和 25°C 下，一单位本品每分钟可以将 1.0 纳摩尔氯霉素和乙酰辅酶 A 转化为氯霉素 3-乙酸酯和辅酶 A。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
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实验方案
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
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