C2900
氯霉素乙酰转移酶 来源于大肠杆菌
lyophilized powder, 50,000-150,000 units/mg protein (Lowry)
别名:
CAT, 乙酰辅酶 A：氯霉素 3-O-乙酰转移酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
50,000-150,000 units/mg protein (Lowry)
form
lyophilized powder
specific activity
50,000-150,000 units/mg protein (Lowry)
mol wt
75 kDa (three identical subunits)
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
Escherichia coli ... cat(2847485)
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General description
The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.
Biochem/physiol Actions
这种酶是细菌对氯霉素产生抗性的原因。催化乙酰辅酶 A 和氯霉素转化为辅酶 A 和氯霉素 3-乙酸酯。
Physical form
部分纯化；含 Tris 缓冲盐。
Other Notes
在 pH 7.8 和 25°C 下，一单位本品每分钟可以将 1.0 纳摩尔氯霉素和乙酰辅酶 A 转化为氯霉素 3-乙酸酯和辅酶 A。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Fang Wang et al.
Gene, 500(1), 59-62 (2012-04-05)
Numerous matrix attachment regions (MARs) have been used to improve transgene expression in genetic engineering, but an efficient and stable expression vector is lacking. In the present study, a vector named pCCF containing chloramphenicol acetyltransferase (CAT) reporter gene cassettes was
Bédis Dridi et al.
The Journal of antimicrobial chemotherapy, 66(9), 2038-2044 (2011-06-18)
Methanogenic archaea are constant members of the human oral and digestive microbiota retrieved, in particular, from periodontitis lesions. The objective of the study was to determine their susceptibility to antimicrobials. Using the macrodilution method in Hungate tubes with optical microscope
Junbo Liu et al.
FEBS letters, 586(12), 1719-1723 (2012-05-16)
Bicoid (Bcd) is a Drosophila morphogenetic protein and a transcriptional activator. Genetic studies have suggested a role of sumoylation in Bcd function, but it is unknown how Bcd activity is affected specifically by its own sumoylation status. Here we show
Hirofumi Nariya et al.
Applied and environmental microbiology, 77(23), 8439-8441 (2011-10-04)
A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo
Tapan Biswas et al.
Protein science : a publication of the Protein Society, 21(4), 520-530 (2012-02-02)
Novel antibiotics are needed to overcome the challenge of continually evolving bacterial resistance. This has led to a renewed interest in mechanistic studies of once popular antibiotics like chloramphenicol (CAM). Chloramphenicol acetyltransferases (CATs) are enzymes that covalently modify CAM, rendering
实验方案
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
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