C2905
软骨素酶 ABC 来源于普通变形杆菌
lyophilized powder, 0.3-3 units/mg solid
别名:
Chondroitin ABC Lyase
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-777-9
MDL number:
Specific activity:
0.3-3 units/mg solid
Biological source:
bacterial (Proteus vulgaris)
biological source
bacterial (Proteus vulgaris)
Quality Level
conjugate
(Glucosaminoglycan)
form
lyophilized powder
specific activity
0.3-3 units/mg solid
mol wt
~120 kDa by gel filtration
composition
Protein, ~35% biuret
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
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Application
Sigma的软骨素酶ABC被用来消化O连接的硫酸软骨素类糖胺聚糖(CSGAG)。该酶还被用来消化蛋白质多糖中的糖胺聚糖链，在哺乳动物系统中构建和表达重组的多功能蛋白聚糖基因。此外，它还被用来消化蛋白聚糖复合物中的硫酸软骨素。该酶还被用于从蛋白聚糖中去除糖链，从而消除蛋白聚糖的轴突生长抑制作用。
Biochem/physiol Actions
软骨苷酶ABC催化含有(1-4)-β-D-己糖氨基和(1-3)-β-D-葡萄糖醛酸基(或(1-3)-α-L-iduronosyl)的多糖与含有4-脱氧-β-D-葡萄糖-4-enuronosyl基团的双糖的清除降解。它作用于硫酸软骨素4、硫酸软骨素6、硫酸皮肤素。但是，它对透明质酸盐作用缓慢。其分子量约为120 kDa，含分子量分别为86 kDa和32 kDa的两个不同亚单位。硫酸软骨素pH值为8.0，透明质酸pH值为6.8。最佳温度为37℃。1mM Zn2+ 可作为其抑制剂，0.05 M乙酸为该酶的激活剂。
Packaging
包装按照软骨素酶C活性。
Physical form
含 Tris 缓冲盐的冻干粉
Preparation Note
在0.01%牛血清白蛋白水溶液(BSA)中复溶。随后的稀释液可以制成pH 8.0的含有50mm Tris,60mm乙酸钠和0.02% BSA的缓冲液。溶液需新鲜配制。
Other Notes
在37°C、pH 8.0条件下，一单位每分钟将释放1.0 μmole的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖)-4-烯-吡喃糖醛糖醛酸）-4-O-磺基-D-半乳糖的混合物和1.0 μmole来自鲨鱼软骨硫酸软骨素的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖-4-烯-吡喃糖醛糖醛酸)-6-O-磺基-D-半乳糖。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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商品
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C2905-10UN | 04061833484845 |
| C2905-2UN | 04061833484852 |
| C2905-5UN | 04061833484869 |
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