产品名称
碳酸盐-碳酸氢盐缓冲液, capsule
form
capsule
Quality Level
color
colorless
pH
9.3-9.9
solubility
water, high purity: 1 capsule/100 mL
application(s)
diagnostic assay manufacturing
Application
制备的缓冲液适于在多孔板或塑料管上进行蛋白包被。
碳酸盐-碳酸氢盐缓冲液已用于悬浮冻干人血浆样品，以进行微量滴定板生物膜测定和流通池生物膜测定。在酶联免疫吸附测定(ELISA)之前，还可将其用作微量滴定板的包被缓冲液。
General description
碳酸氢盐缓冲液的pH值大多接近7.4。它是主要的小肠缓冲液。
Other Notes
Preparation Note
用100mL去离子水完全溶出胶囊并使之溶解。一个胶囊的含量为100ml的0.05 M的碳酸氢盐缓冲液，在25℃下，pH 9.6。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Evolution of a physiological pH 6.8 bicarbonate buffer system: application to the dissolution testing of enteric coated products
Liu F, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 78(1), 151-157 (2011)
T J Caperna et al.
Domestic animal endocrinology, 60, 75-82 (2017-05-30)
A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen.
Quantifying and comparing constitutive immunity across avian species
Millet S, et al.
Developmental and Comparative Immunology, 31(2), 188-201 (2007)
Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
von Gersdorff J, et al.
Journal of Fish Diseases, 34(10), 749-755 (2011)
R M Jaafar et al.
Journal of fish diseases, 41(1), 117-123 (2017-07-15)
Teleosts are able to raise a protective immune response, comprising both innate and adaptive elements, against various pathogens. This is the basis for a widespread use of vaccines, administered as injection or immersion, in the aquaculture industry. It has been
实验方案
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。
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