C3117
Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse
~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution
别名:
Monoclonal Anti-Cy3/Cy5
生物来源
mouse
质量水平
偶联物
biotin conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
CY-96, monoclonal
表单
buffered aqueous solution
浓度
~1 mg/mL
技术
direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable
同位素/亚型
IgG2a
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
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一般描述
Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.
免疫原
mixture of proteins labeled with Cy3/Cy5.
应用
Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- dot blot assay
- protein microarrays
- immunofluorescenc
Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.
生化/生理作用
Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
The antibody recognizes Cy3 and Cy5 conjugated to proteins.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Wei Li et al.
Animal models and experimental medicine (2023-08-11)
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Frontiers in immunology, 14, 1149810-1149810 (2023-04-11)
Patients with diabetes mellitus (DM) have a higher incidence of malignant tumors than people without diabetes, but the underlying molecular mechanisms are still unclear. To investigate the link between DM and cancer, we screened publicly available databases for diabetes and
Chao-Yue Yang et al.
Chinese journal of traumatology = Zhonghua chuang shang za zhi, 26(3), 155-161 (2023-04-06)
This study aims to elucidate the electrotaxis response of alveolar epithelial cells (AECs) in direct-current electric fields (EFs), explore the impact of EFs on the cell fate of AECs, and lay the foundation for future exploitation of EFs for the
Quantitative profile of the uropathogenic Escherichia coli outer membrane proteome during growth in human urine
Alteri CJ and Mobley HLT
Infection and Immunity, 75(6), 2679-2688 (2007)
Aereas Aung et al.
Science (New York, N.Y.), 379(6630), eabn8934-eabn8934 (2023-01-27)
The structural integrity of vaccine antigens is critical to the generation of protective antibody responses, but the impact of protease activity on vaccination in vivo is poorly understood. We characterized protease activity in lymph nodes and found that antigens were
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