C3131
线粒体蛋白 来源于牛心脏
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture
别名:
CYC, CytC
生物来源
bovine heart
质量水平
方案
≥95% based on Mol. Wt. 12,327 basis
表单
powder
分子量
12327 Da
储存条件
(Tightly closed Dry)
技术
cell culture | mammalian: suitable
杂质
0.40-0.50% Iron (anhydrous)
溶解性
water: 10 mg/mL, dark red-brown
UniProt登记号
储存温度
−20°C
基因信息
cow ... CYC1(512500)
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一般描述
研究领域：细胞凋亡
细胞色素C(Cyt C)是重要的线粒体蛋白质，位于线粒体膜内叶。在细胞核中编码为apo-细胞色素C。细胞色素C结合在膜上，或作为能量传递膜中的可溶性金属蛋白，存在于细菌、古菌和质体中。属于IIa类细胞色素，含有五配位血红素中心。
细胞色素C(Cyt C)是重要的线粒体蛋白质，位于线粒体膜内叶。在细胞核中编码为apo-细胞色素C。细胞色素C结合在膜上，或作为能量传递膜中的可溶性金属蛋白，存在于细菌、古菌和质体中。属于IIa类细胞色素，含有五配位血红素中心。
应用
细胞色素C（来自牛心）已用于：
- 作为检测溶液组分，检测人骨肉瘤和人肝癌细胞线粒体中呼吸链复合体III和IV的活性
- 测定人主动脉内皮细胞线粒体中细胞色素C氧化酶的活性
- 作为细胞色素C氧化酶反应液组分，用于小鼠肾组织中细胞色素C氧化酶的原位染色
生化/生理作用
在细胞内，细胞色素c可在亚铁态和三价铁态之间迅速转化，这使其成为有效的生物电子转运体。它在动植物的细胞氧化过程中发挥重要作用。它通常被视作呼吸作用的通用催化剂，可在呼吸底物与氧气之间形成必要的电子桥。
细胞色素C(Cyt C)在caspase依赖性内源性凋亡途径参与激活caspase，通过凋亡启动细胞程序性死亡。具有脂结合活性，与心磷脂的相互作用与超氧化酶活性有关。细胞色素C在细胞色素C血红素裂解酶的帮助下结合血红素 ，释放到线粒体膜间隙中。
细胞色素c可在亚铁态和三价铁态之间迅速转化，这使其成为有效的生物电子载体。它在动植物的细胞氧化过程中发挥重要作用。通常视作是呼吸链中的通用连接，它在可呼吸底物与氧气之间形成必要的电子桥。
制备说明
醋酸制备，不需要TCA。
其他说明
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Stéphane T Gabilly et al.
Frontiers in plant science, 8, 1313-1313 (2017-08-12)
Cytochromes c are hemoproteins, with the prosthetic group covalently linked to the apoprotein, which function as electron carriers. A class of cytochromes c is defined by a CXXCH heme-binding motif where the cysteines form thioether bonds with the vinyl groups
C Garrido et al.
Cell death and differentiation, 13(9), 1423-1433 (2006-05-06)
In healthy cells, cytochrome c (Cyt c) is located in the mitochondrial intermembrane/intercristae spaces, where it functions as an electron shuttle in the respiratory chain and interacts with cardiolipin (CL). Several proapoptotic stimuli induce the permeabilization of the outer membrane
Increasing cGMP-dependent protein kinase I activity attenuates cisplatin-induced kidney injury through protection of mitochondria function
Hasiyeti M, et al.
American Journal of Physiology: Renal Physiology (2013)
Yong-Ling P Ow et al.
Nature reviews. Molecular cell biology, 9(7), 532-542 (2008-06-24)
Cytochrome c is primarily known for its function in the mitochondria as a key participant in the life-supporting function of ATP synthesis. However, when a cell receives an apoptotic stimulus, cytochrome c is released into the cytosol and triggers programmed
Marina L Pridatchenko et al.
Analytical and bioanalytical chemistry, 402(8), 2521-2529 (2011-09-09)
The amino acid sequence determines the individual protein three-dimensional structure and its functioning in an organism. Therefore, "reading" a protein sequence and determining its changes due to mutations or post-translational modifications is one of the objectives of proteomic experiments. The
商品
Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.
实验方案
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
Chromatograms
application for HPLC
application for HPLC
application for HPLC
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