C3138
组织蛋白酶D 来源于牛脾脏
lyophilized powder, ≥2.0 units/mg protein
别名:
CTSD
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
≥2.0 units/mg protein
Assay:
10—70% protein (biuret)
Biological source:
bovine spleen
biological source
bovine spleen
assay
10—70% protein (biuret)
form
lyophilized powder
specific activity
≥2.0 units/mg protein
mol wt
~45 kDa
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
dark brown
suitability
suitable for molecular biology
UniProt accession no.
application(s)
life science and biopharma
storage temp.
−20°C
Quality Level
Gene Information
cow ... CTSD(282883)
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General description
研究领域：细胞信号传导
组织蛋白酶D是可溶性内涵体-溶酶体天冬氨酸蛋白酶，在粗面内质网中以前体酶原(preprocathepsin D)形式合成。由11p15.5区的CTSD基因编码。
组织蛋白酶D是可溶性内涵体-溶酶体天冬氨酸蛋白酶，在粗面内质网中以前体酶原(preprocathepsin D)形式合成。由11p15.5区的CTSD基因编码。
Application
组织蛋白酶D（来自牛脾）已用于：
- 体外剂量依赖性荧光活性检测。
- 消化体外髓鞘少突胶质细胞糖蛋白(MOG)，以研究丙二醛(MDA)修饰MOG的摄取及其对中枢神经系统自身免疫的意义。
- 酶促消化关节软骨的蛋白聚糖部分，以测定两种不同组织结构水平下的软骨弹性模量。
- 组织蛋白酶D活性检测。
Biochem/physiol Actions
核内体-溶酶体天冬氨酸蛋白酶，与乳腺癌转移和阿尔茨海默病有关。 人们发现，在凋亡的人包皮成纤维细胞中，溶酶体释放组织蛋白酶D先于释放细胞色素c和膜电位丧失。
组织蛋白酶D是一种核内体-溶酶体天冬氨酸蛋白酶，与乳腺癌转移和阿尔茨海默病有关。 人们发现，在凋亡的人包皮成纤维细胞中，溶酶体释放组织蛋白酶D先于释放细胞色素c和膜电位丧失。 诱导凋亡后12至24小时，PC12细胞中的组织蛋白酶D水平升高。
Physical form
含有柠檬酸盐缓冲液盐的冻干粉
Other Notes
在pH 3.0、37 °C条件下，一个单位可在一分钟内使一毫升溶液的A280增加1.0（以血红蛋白为底物，对TCA可溶性产物进行测定（1 cm光程））。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
动植物源性产品
此项目有
Olja Mijanovic et al.
Pharmaceutics, 13(6) (2021-07-03)
Lysosomal proteases play a crucial role in maintaining cell homeostasis. Human cathepsin D manages protein turnover degrading misfolded and aggregated proteins and favors apoptosis in the case of proteostasis disruption. However, when cathepsin D regulation is affected, it can contribute
Martin Stolz et al.
Biophysical journal, 86(5), 3269-3283 (2004-04-28)
Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was developed to measure the dynamic elastic modulus, |E(*)|, in compression by indentation-type
Cathepsin D--many functions of one aspartic protease
Benes P, et al.
Critical Reviews in Oncology/Hematology, 68(1), 12-28 (2008)
Marie Maynadier et al.
Journal of controlled release : official journal of the Controlled Release Society, 171(2), 251-257 (2013-08-01)
Implication of the intracellular proteolytic activity of Cathepsin D (CathD), a lysosomal aspartyl-protease overexpressed in numerous solid tumors, has been evidenced on tumor growth. Its intracellular inhibition by potent inhibitors such as pepstatin constitutes a relevant but challenging molecular target.
Yoko Shiba et al.
Current biology : CB, 23(19), 1945-1951 (2013-10-01)
ArfGAPs are known to be involved in cargo sorting in COPI transport. However, the role of ArfGAPs in post-Golgi membrane traffic has not been defined. To determine the function of ArfGAPs in post-Golgi traffic, we used small interfering RNA to
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