C3355
Coelenterazine hcp
≥95% (HPLC), solid
别名:
CLZN-hcp
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关于此项目
经验公式（希尔记法）:
C25H25N3O2
化学文摘社编号:
分子量:
399.48
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
产品名称
Coelenterazine hcp, solid
方案
≥95% (HPLC)
质量水平
表单
solid
溶解性
methanol: 1 mg/mL, clear, yellow to orange
储存温度
−20°C
SMILES字符串
Oc1ccc(cc1)C2=CN3C(=O)C(Cc4ccccc4)=NC3=C(CC5CCCC5)N2
InChI
1S/C25H25N3O2/c29-20-12-10-19(11-13-20)23-16-28-24(21(26-23)14-17-8-4-5-9-17)27-22(25(28)30)15-18-6-2-1-3-7-18/h1-3,6-7,10-13,16-17,26,29H,4-5,8-9,14-15H2
InChI key
YEGCUOQUFAXCMK-UHFFFAOYSA-N
一般描述
Coelenterazine hcp is a semisynthetic analog of coelenterazine.
应用
Coelenterazine hcp has been used:
- as a component of coelenterazine reagent in the reporter assay
- in the purification of Renilla luciferase-labeled Annexin V
- in evaluating the activity of aequorin in Escherichia coli
生化/生理作用
Coelenterazine hcp has 190 times higher luminescence intensity than native coelenterazine and a faster response time than native coelenterazine.
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
Mehdi Zeinoddini et al.
Photochemistry and photobiology, 86(5), 1071-1075 (2010-07-29)
An aequorin-based Escherichia coli strain JM109 biosensor was constructed and characterized for its potential to detect toluene and related compounds in aqueous solutions. The biosensor was constructed based on a PGL2 plasmid carrying the lower pathway promoter (Pu) of the
Sarah E Webb et al.
Molecular reproduction and development, 82(7-8), 563-586 (2014-01-03)
In this review, we take a retrospective look at the discovery and utilization of the Ca(2+) -sensitive bioluminescent protein complex, aequorin. We do consider the contribution it has made to our understanding of the natural phenomenon of bioluminescence, but it
Mahboobeh Nazari et al.
The Analyst, 137(21), 5062-5070 (2012-09-25)
The Ca(2+)-dependent binding of Annexin V to phosphatidylserine on cell surfaces is a reliable marker for apoptosis that is widely used in flow cytometry based apoptosis assays. In this paper, we report a new class of Annexin V-based probes for
Yao Cheng et al.
Plant & cell physiology, 59(5), 978-988 (2018-02-13)
Seed germination is sensitive to salt stress. ABA and Ca2+ are involved in the regulation of seed germination under salt stress. Ca2+ influx mediated by glutamate receptors (GLRs) plays important roles in many physiological processes in plants. Here, we investigated
Hui Zhao et al.
Molecular imaging, 3(1), 43-54 (2004-05-15)
In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes, luciferases, and delivery of chemical substrates to expressing cells. Coelenterazine (CLZN) is the substrate for a group of bioluminescent enzymes obtained from marine organisms. At present, there are
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