Merck
CN
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文件

C3355

Sigma-Aldrich

Coelenterazine hcp

solid

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别名:
CLZN-hcp
经验公式（希尔记法）:
C25H25N3O2
CAS号:
123437-32-1
分子量:
399.48
MDL编号:
MFCD03453204
PubChem化学物质编号:
24892609
NACRES:
NA.83

检测方案

≥95% (HPLC)

质量水平

200

形式

solid

溶解性

methanol: 1 mg/mL, clear, yellow to orange

储存温度

−20°C

SMILES字符串

Oc1ccc(cc1)C2=CN3C(=O)C(Cc4ccccc4)=NC3=C(CC5CCCC5)N2

InChI

1S/C25H25N3O2/c29-20-12-10-19(11-13-20)23-16-28-24(21(26-23)14-17-8-4-5-9-17)27-22(25(28)30)15-18-6-2-1-3-7-18/h1-3,6-7,10-13,16-17,26,29H,4-5,8-9,14-15H2

InChI key

YEGCUOQUFAXCMK-UHFFFAOYSA-N

相关类别

一般描述

Coelenterazine hcp is a semisynthetic analog of coelenterazine.

应用

Coelenterazine hcp has been used:
  • as a component of coelenterazine reagent in the reporter assay
  • in the purification of Renilla luciferase-labeled Annexin V
  • in evaluating the activity of aequorin in Escherichia coli

生化/生理作用

Coelenterazine hcp has 190 times higher luminescence intensity than native coelenterazine and a faster response time than native coelenterazine.

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315 - H319 - H335

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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Hui Zhao et al.
Molecular imaging, 3(1), 43-54 (2004-05-15)
In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes, luciferases, and delivery of chemical substrates to expressing cells. Coelenterazine (CLZN) is the substrate for a group of bioluminescent enzymes obtained from marine organisms. At present, there are
Xingfeng Liu et al.
Methods in molecular biology (Clifton, N.J.), 1344, 275-286 (2015-11-02)
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An aequorin-based Escherichia coli strain JM109 biosensor was constructed and characterized for its potential to detect toluene and related compounds in aqueous solutions. The biosensor was constructed based on a PGL2 plasmid carrying the lower pathway promoter (Pu) of the
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