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Merck
CN

C3921

Creatinase from Pseudomonas sp.

recombinant, expressed in E. coli, lyophilized powder, 10-15 units/mg protein

别名:

Creatine Amidinohydrolase

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-471-1
MDL number:
Specific activity:
10-15 units/mg protein
Recombinant:
expressed in E. coli
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recombinant

expressed in E. coli

form

lyophilized powder

specific activity

10-15 units/mg protein

mol wt

45 kDa

composition

Protein, ~70% biuret

storage temp.

2-8°C

Application

Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.

Biochem/physiol Actions

Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H2O, to form urea and sarcosine.

Other Notes

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

存储类别

11 - Combustible Solids

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
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分析证书(COA)

Lot/Batch Number

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M-L Lee
Pediatric cardiology, 26(6), 792-796 (2005-08-06)
I report on a 3-month-old infant with pulmonary atresia-intact ventricular septum and ventriculocoronary communication (VCC) who underwent percutaneous radiofrequency valvulotomy and valvuloplasty (RFVV). The patient's cardiac troponin-I, creatine kinase (CK), and myocardial fraction of (CK-MB) were elevated before RFVV and
Jill A O'Loughlin et al.
ASAIO journal (American Society for Artificial Internal Organs : 1992), 50(3), 253-260 (2004-06-03)
An alginate microcapsule was developed that contains three enzymes (urease, uricase, and creatininase) capable of effectively degrading urea, uric acid, and creatinine, which are elevated to pathologic levels in patients with kidney failure. The capsules were evaluated in vitro and
Ashutosh Tiwari et al.
International journal of biological macromolecules, 44(5), 408-412 (2009-05-12)
A matrix made up of chitosan-SiO(2)-multiwall carbon nanotubes (CHIT-SiO(2)-MWCNTs) nanocomposite was fabricated to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-SiO(2)-MWCNTs matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-SiO(2)-MWCNTs biomatrix was characterized
Selective embolization in the management of arteriovenous fistula after renal allograft biopsy preserves renal allograft function.
Galip Guz et al.
International urology and nephrology, 37(1), 207-208 (2005-09-01)
Emine Karakuş et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 34(3), 337-347 (2006-07-01)
New potentiometric and amperometric biosensors were developed for the determination of creatine. The potentiometric creatine biosensor was prepared by immobilizing urease and creatinase on poly(vinylchloride) (PVC) ammonium membrane electrode containing palmitic acid prepared by using nonactine as an ammonium-ionophore. The

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